超滤法测定丹酚酸A的血浆蛋白结合率

Determination of Plasma Protein Binding Rate of Salvianol Acid A by Ultrafiltration

目的:建立血浆样品中丹酚酸A浓度的分析方法,并测定丹酚酸A的血浆蛋白结合率,为进一步展开丹酚酸A的代谢和药动学研究及指导临床用药提供参考。方法:采用超滤法和HPLC测定丹酚酸A在牛血清白蛋白(BSA),大鼠血浆,新西兰兔血浆和比格犬血浆中的蛋白结合率。结果:丹酚酸A在0.01～2.5 mg.L-1线性良好,标准曲线方程为Y=0.807 3X+0.013 2(r=0.999 4)。丹酚酸A与BSA,大鼠血浆,新西兰兔血浆和比格犬血浆在5.0,50.0,100.0 mg.L-1的含药血浆中其平均蛋白结合率分别为(99.79±0.02)%,(99.79±0.03)%,(99.73±0.06)%,(99.81±0.03)%。结论:所建立的方法灵敏度高,专属性和重复性好,操作简单,能够满足定量分析测试要求。丹酚酸A与血浆蛋白有很强的结合,且在已考察的血药浓度范围内其血浆蛋白结合率无明显浓度依赖性和种属差异性。

Objective： To establish a method for the determination of salvianolic acid A in the plasma samples, and then study the plasma protein binding rate of salvianolic acid A. This can provide a reference to the further study of salvianol acid A in its metabolism, pharmacokinetics and clinical treatment. Method： The ultrafihration was employed to determine the plasma protein binding rate of salvianol acid A in BSA plasma samples, rat plasma samples, the New Zealand rabbit plasma samples and beagle dog plasma samples. The plasma concentrations of salvianol acid A were measured by RP-HPLC. Result： The calibration curve of salvianolic acid A was linear within the ranges of 0.01-2.5 mg .L-1 （r =0. 999 4）. The average plasma protein binding rates of salvianol acid A with BSA, rat plasma, the New Zealand rabbit plasma and beagle dog plasma were （99.79 ± 0.02）%, （99.79 ±0.03）%, （99.73 ±0.06）%, （99.81 ±0.03）% in the plasma concentrations of 5.0, 50.0, 100.0 mg · L-1 respectively. Conclusion： The method has high sensitivity, good specificity and reproduction, with simple management thus fulfilling the requirement. Salvianol acid A shows a high binding power to plasma protein. , and this was independent of the investigated concentrations and the different species.