大蒜多糖对甲醛所致人胚肝细胞损伤的保护作用

Protective effect of garlic polysaccharide on human embryo hepatocytes damaged by formaldehyde

目的探讨大蒜多糖对甲醛致人胚肝细胞L02氧化损伤和遗传损伤的保护作用。方法采用体外常规培养L02,取对数生长期的细胞进行实验。实验设对照组、甲醛组和低、中、高剂量大蒜多糖保护组,其中大蒜多糖先与细胞预孵8h后加入0.1mmoL/L甲醛,置CO2培养箱培养4h,用单细胞琼脂糖凝胶电泳评价大蒜多糖各浓度实验组处理L02细胞4hDNA的损伤程度;检测细胞培养液中SOD和GSH活力及LDH、MDA和AST的含量。结果大蒜多糖各保护组与甲醛组比较,Tail Moment和Tail DNA都明显减少,并与大蒜多糖浓度增加呈剂量一效应关系,其差异均有统计学意义(P<0.05);SOD和GSH-Px活力明显升高,LDH、AST和MDA含量均降低(P<0.05)。结论大蒜多糖可提高L02细胞抗氧化酶的活性,减轻甲醛所致的氧化损伤和遗传损伤。

Objective To investigate the protective effects of garlic polysaccharide on oxidative damage and genetic injury of human embryo hepatocytes （ L02 ） caused by formaldehyde. Methods L02 cells were conventionally cultured with 1640 medium in vitro, and the cells at exponential growth phase were selected to be experimented. The experimental group included control group, formaldehyde group, low-dose, medium-dose, high-dose garlic polysaccharide groups. L02 cells were cultured with garlic polysaccharide for 8h in advance, then 0.1 mmol/L formaldehyde,placed in CO2 incubator for 4h. The DNA injury degree of ID2 cells 4h after exposure to formaldehyde was evaluated by monoplast agarose gel electrophoresis, and the activities of SOD and GSH in cell culture fluid were detected, and the contents of LDH, MDA and AST were measured. Results As compared with those in formaldehyde group, Tail Moment and Tail DNA of L02 cells in the three doses groups of garlic polysaccharide were significantly decreased, furthermore, which was in a dose-dependent way, there were significant differences among the groups（ P 〈0.05）. The activities of SOD and GSH-Px were obviously increased,however, the contents of LDH, AST and MDA were significantly decreased （ P 〈 0. 05 ）. Conclusion The garlic polysaccharide can enhance the activity of antioxidant enzymes of L02 cells, as a result, which can reheve the oxidative damage and genetic injury caused by formaldehyde.