慢性乙肝、肝硬化和肝癌患者Th1/Th2细胞因子检测及临床应用

Clinical Application of Th1/Th2 Cytokines in Chronic Hepatitis B (CHB), Liver Cirrhosis and HCC Patients Liver Cirrhosis and HCC Patients

目的:应用流式微球技术(cytometric bead array,CBA)技术检测慢性乙肝、肝硬化及肝细胞性肝癌(hepatic cellular cancer,HCC)患者血浆中Th1/Th2细胞因子水平,探讨其临床应用。方法:选择慢性HBV感染者71例,HBV阳性肝硬化患者33例,HBV阳性肝癌患者25例,用CBA方法检测其血清中Th1类细胞因子TNF-α、IFN-γ、IL-2,Th2类细胞因子IL-4、IL-5、IL-10的表达情况。结果:肝癌患者血清中Th1类细胞因子TNF-α、IFN-γ、IL-2,Th2类细胞因子IL-5表达显著高于慢性乙肝和肝硬化患者,而IL-4、IL-10在三组患者中的表达无显著性差异。结论:肝癌患者机体强势分泌Th1类细胞因子,处于高免疫反应状态。检测乙肝患者Th1/Th2类细胞因子对于肝病慢性化的进展成为一项新的临床预测指标。

Objective To detect plasma Thl/Th2 cytokines in CHB, liver cirrhosis and HCC patients with CBA methods, to explore the associated clinical value. Methods CBA method was used to detect Thl cytokines （TNF-α, IFN-γ, IL-2）, Th2 cytokines （IL-4,IL-5 ,IL-10） in 71 CHB patients, 33 HBV positive liver cirrhosis patients and 25 HBV positive HCC patients. Results The expression of Thl cytokines （TNF-α,IFN-γ/,IL-2） and IL-5 were significantly higher in HCC patients than in CI-IB and liver cirrhosis patients （P 〈 0.01 - 0.05）, the expression of IL-4 and IL-10 showed no difference in three groups of patients. Conclusion High expression of Thl cytokines in malignant hepatoma patients indicates it is high immunological response in these patients. Detection of Thl/Th2 cytokines may be a new clinical predictive indicator for the progress of chronic liver disease.