挤压膨化参数对转基因大豆外源基因降解的影响

The Effect of Extrusion Parameters on the Degradation of Exogenous DNA of GM Soybean

采用定性PCR检测技术,分析了不同挤压腔温度、物料水分和螺杆、喂料器转速的组合工艺条件下,挤压膨化对转基因大豆外源基因CP4-EPSPS的影响。研究结果表明,挤压膨化加工是降解转基因大豆外源基因CP4-EPSPS的有效手段。挤压膨化参数中,对外源基因降解作用由强到弱的顺序为:挤压腔温度>物料水分>螺杆转速或喂料器转速。本试验条件下的最佳挤压膨化参数组合为:挤压腔温度130℃,物料水分20%,螺杆转速72 r/min,喂料器转速88 r/min。在此条件下,大豆外源基因片段可降至206 bp以下而未能检出。

By using qualitative PCR detection method,this experiment analyzed the effects of different extrusion parameter combination of extrusion barrel temperatures, material water contents, the rotate speed of screw and feeder on the degradation of exogenous gene CP4 - EPSP of GM soybean. The results show that extrusion processing is the effective way for the degradation of the foreign CP4 - EPSP gene of GM soybean. In the extrusion processing parameters, the se- quence from strong to weak to impact the degradation of foreign gene is that ： Extrusion barrel temperature 〉 material wa- ter content 〉 screw speed or feeder speed. The optimum extrusion parameter combination is that ： Extrusion barrel tem- perature 130 ℃ ,material water content 20% ,screw speed 72 r/min,feeder speed 88 r/min for this experiment. Under this condition,the CP4-EPSPS gene of GM soybean can be degraded to smaller than 206 bp which can not be detected out.