8种油茶蒲提取物中活性物质含量及其抗氧化能力的比较研究

Comparative Study on Contents of Bioactive Substance and Antioxidant Activities of Eight Kinds of Camellia Fruit Hull Extracts

采用分光光度法测定8种油茶蒲提取物中活性物质含量,结果表明8种油茶蒲提取物中活性物质含量都比较高,尤以普通油茶茶蒲含量最高(多糖含量>317 mg/g提取物,黄酮含量>35 mg/g提取物,皂苷含量>292 mg/g提取物,多酚含量>252 mg/g提取物)。油茶蒲提取物具有较强清除DPPH自由基能力(IC50<112μg/mL)及较高总抗氧化能力,通过相关性分析,发现油茶蒲提取物抗氧化能力与其多酚及多糖类物质含量具有显著相关性。采用高效液相色谱法分析测定8种油茶蒲提取物酚类物质组成,结果显示没食子酸、3-O-甲基鞣花酸-4'-O-β-D-吡喃葡萄糖(MEAG)、鞣花酸为主要特征化合物。活性物质含量及抗氧化能力测定结果表明,油茶蒲提取物有望作为一种天然抗氧化剂运用于食品、保健品及化妆品中。

The bioactive substance contents of eight kinds of Camellia fruit hull extracts from the major regions of China was determined by spectrophotometry, and results showed that Camellia fruit hull extracts was abundant in bioactive substance. Remarkable high phenolic content （ gallic acid equivalent, GAE 〉 252 mg/g） , polysaccharides content（glucose equivalent, GE 〉 317 mg/g）, saponin content （ginsenoside Re, GR 〉 292 mg/g）, flavonoid content （ rutin equivalent, RE 〉 35 rag/g） was found in samples of Camellia oleifera Abel. The Camellia fruit hull extracts also demonstrated strong DPPH radical-scavenging ability（ IC50 〈 112 μg/mL）and high total antioxidant ability; mean-while a significant correlation was observed between antioxidant abilities and the content of phenolic compounds and polysaccharides of respective extracts. Phenolic compounds of extracts were analyzed by HPLC to find that gallic acid/ MEAG/ellagic acid played the predominant roles in phenolic compounds comoared with the standard substances. The above results suggest that extracts of Camellia fruit hull can be applied in food/health care/cosmetic industry as a source of natural antioxidant.