核因子-κB在重组人促红细胞生成素预防神经病理性疼痛中的作用

The role of NF-κB in the process of recombinant human erythropoietin preventing neuropathic pain

目的探讨脊髓核因子(NF)-κB在重组人促红细胞生成素(recombinant human erythropoietin,rhEPO)预防神经病理性疼痛中的作用机制。方法选用SD雄性大鼠30只,制作L5脊神经切断神经病理性模型,随机分为三组:假手术组(C组)、生理盐水组(S组)和rhEPO组(E组),S组和E组分别从术前1d开始腹腔注射生理盐水和rhEPO 5kU/kg,连续7d。采用von Frey和Hargreaves法测定大鼠术前、术后3d和7d机械缩足反射阈值(MWT)和热缩足潜伏期(TWL);采用EMSA法检测术后7d脊髓NF-κB表达水平。结果与C组相比,术后3、7dS组和E组大鼠术侧MWT和TWL明显下降(P<0.01),且S组明显低于E组(P<0.01)。与C组相比,术后7dS组和E组腰段脊髓NF-κB的活性明显增高(P<0.01),且S组明显高于E组(P<0.01)。结论 rhEPO能减轻神经病理性疼痛的发生,其效应可能与其减少脊髓NF-κB的活性有关。

Objective To investigate the role of NF-（B in the process of recombinant human erythropoietin（rhEPO） preventing neuropathic pain. Methods Thirty male Sprague-Dawley rats were randomly assigned into three groups with each group ten rats： control group （group C）, saline group （group S）, and rhEPO group （group E）. Rats in group S and group E were intraperitoneally injected with saline or rhEPO 5 kU/kg 1 day before surgery and continued daily to ？ days. The hind paw mechanical withdrawal threshold （MWT） and thermal withdrawal latency （TWL） were measured before the operation and 3 d and ？ d after the operation with von Frey hairs and Hargreaves tests, respectively. The expression of NF-（B in spinal cord was assessed by electrophoretic mobility shift assay （EMSA）. Results Compared with group C, MWT and TWL decreased and spinal NF-KB expression increased in group S and E （P〈0. 01）. Compared with group S, MWT and TWL increased and spinal NF-KB expression decreased in group E （P〈0.01）. Conclusion rhEPO prevents neuropathic pain, which may be attributed to the reduced spinal expression of NF-KB.