基于典型相关分析的砂仁性状与内在物质基础相关性研究

Correlation between properties and inner material basis of Sharen(Fructus Amomi) based on Cancorr analysis

目的研究中药砂仁性状和内在物质基础的相关性,为砂仁质量标准中性状特征提供试验依据和数据支持。方法以纵径、横径、百果重、种子团每室种子数为性状指标,采用人工测量法进行分析。水蒸气蒸馏法测定挥发油含量,气相色谱法(GC)测定主要挥发性成分乙酸龙脑酯和樟脑的含量;高效液相色谱法(HPLC)和紫外分光光度法(UV)分别对水溶性成分槲皮苷和总黄酮进行含量测定。采用SAS统计分析系统典型相关分析(Cancorr分析)对所得结果进行相关性分析。结果 Cancorr分析的典型相关系数(λ1)为0.961 0。具体分析结果如下:来自性状指标的第一典型变量中种子团每室种子数的权数最大,为-0.208 9;而性状特征在槲皮苷含量上的相关系数最大,为-5.366 8,其余具有一致趋势的相关系数依次为挥发油含量为-1.879 7、乙酸龙脑酯含量为-1.878 9、乙酸龙脑酯与樟脑含量比值为-0.913 6。结论研究结果表明了不同性状砂仁物质基础存在显著性差异,证明了优质砂仁性状特征的科学性与合理性,为进一步完善砂仁的质量评价体系奠定了基础。

Objective To study the correlation between properties and inner material basis of Sharen （Fructus Amomi）, and provide experimental evidences and data supports for quality standard of S haren （properties and characteristics）. Methods Artificial measurement was applied in the analysis taking longitudinal diameter, horizontal diameter, 100-pod weight and seed number per locule of seed group as property indexes. The content of volatile oil, bornyl acetate and camphor were detected respectively by using steam distillation and gas chromatography. The content of quercetin and total flavonoids （water- soluble constituents） were detected respectively by using HPLC and ultraviolet spectrophotometry. The correlation among the results was analyzed by applying Cancorr analysis of SAS system. Results The canonical correlation coefficient （ A1 ） analyzed by Cancorr analysis was 0. 961 0. The specific results were as follows： the weight of seed number per locule of seed group from the first canonical variable of property indexes was the highest （ -0. 208 9 ）, and correlation coefficient of properties and characteristics was the highest （ - 5. 366 8 ） in quercetin content. Other correlation coefficients with coincident trend were successively content of volatile oil （ - 1. 879 7 ）, content of bornyl acetate （ -1. 878 9） and ration of bornyl acetate to camphor （ -0. 913 6）. Conclusion The results indicate thatthere is significant difference in inner material basis among Sharen with different properties, which proves the scientificity and rationality of properties and characteristics of superior Sharen and provides the foundation for improving quality evaluation system of Sharen.