石河子地区棉田棉铃虫种群体内相关酶活及含量的测定

Determination of Contents and Activities of Detoxification Enzymes of Helicoverpa armigera in Field Populations in Shihezi District

【目的】测定石河子地区棉铃虫体内羧酸酯酶、磷酸酯酶、谷胱甘肽-S-转移酶以及多功能氧化酶(MFOs)四类主要解毒代谢酶的含量及活性,并与敏感品系棉铃虫进行比较,为棉铃虫抗药性研究提供实验依据。【方法】利用紫外分光光度计比色法测定酶源蛋白含量及比活力。【结果】石河子地区棉铃虫体内的MFOs的酶原蛋白含量及比活力均高于敏感品系棉铃虫且差异显著,其酶源蛋白含量分别为105.943 167、96.158 47μg/头,比活力(0.485 3±0.002 1)、(0.352 3±0.001 4)μM/μg·30 min,其余三种酶在两品系间差异不显著。【结论】NFOs活性的增加是石河子地区棉铃虫抗药性增强重要因素之一。

[ Objective ] In order to provide experimental data for the research on the insecticide resistance of H. armigera, the contents and activities of the main detoxification enzymes such as carboxylesterase （ Care ）, phosphatase, glutathione S - transferase （GST）, monooxygenase （ MFOs ） of H. arrnigera in field populations in Shihezi district were determined when compared with the susceptible strain of 11. armigera. [ Method] The enzyme protein contents and specific activities were measured by ultraviolet spectrophotometry. [ Result ] The content of MFOs of H. armigera in field populations in Shihezi district was 105. 943 167 μg/insect, distinctly higher than that of susceptible strain which was 96. 158 47 μg/insect. Furthermore, the specific activity of the former was 0. 485 3 ±0.002 1 μM/μg · 30 min, also higher than that of susceptible strain which was 0.352 3 ±0. 001 4 μM/μg · 30 min. There were no significant differences of the contents and activities of the other three enzymes between field populations in Shihezi district and the susceptible strain. [ Conclusion ] The increased activity of MFOs was one of the main factors contributing to the enhanced resistance to insecticides of H. armigera in field populations in Shihezi district.