蝉花虫草中核苷类成分的分离纯化和鉴定

Purification and Identification of Nucleoside Components in Cordyceps cicadae Fruit Bodies

对蝉花虫草子实体中的核苷类成分进行分离纯化,并对获得的组分进行鉴定。结果显示:采用65%乙醇超声波法提取的蝉花虫草子实体提取物于10000r/min离心5min后,经反相HPLC制备液相制备(色谱条件为:流动相为0～100%甲醇,梯度洗脱,洗脱速率为15mL/min,进样量1mL,检测波长260nm),分离得到6种主要化合物,经鉴定,6种化合物均为核苷类产品,分别为胞嘧啶、尿苷、肌苷、鸟苷、腺苷和虫草素。对6种化合物进行纯度验证,结果表明,6种化合物的纯度均在95%以上,说明采用反相HPLC分离制备蝉花虫草中的核苷类成分方法是可行的,并且该方法简便、准确。

The aim of this study was to extract, purify and identify nucleoside components of Cordyceps cicadae fruit bodies. Samples were extracted with 65% ethanol by ultrasonic-assisted extraction, and a supernatant was obtained from the resulting extract by centrifugation at 10000 r/min for 5 min and separated by RP-HPLC using 0-100% methanol as a mobile phase through gradient elution at a flow rate of 15 mL/min （1 mL injection volume, 260 nm detection wavelength）. As a result, 6 final products were obtained and identified as cytosine, uridine, inosine,guanosine, adenosine and cordycepin, respectively, with a purify exceeding 95%. This study demonstrates that RP-HPLC is a feasible, simple and accurate technique for separating and preparing nucleoside components of Cordyceps cicadae fruit bodies.