中心组合设计优化热带假丝酵母菌冷冻干燥保护剂

Optimization of Cryoprotectant Formulation for Candida tropical by Central Composite Design

采用单因素试验和响应面中心组合设计,对热带假丝酵母菌真空冷冻干燥保护剂配方进行筛选和优化。通过Design-Expert软件对试验数据进行回归分析,得到保护剂最佳配方为:蔗糖14.15g/100mL、L-谷氨酸钠7.07g/100mL、聚乙二醇1.10g/100mL,菌体存活率预测值为81.46%,实际值为82.73%。

A cryoprotectant formulation consisting of sucrose, L-sodium glutamate and polyethylene glycol for Candida tropical was optimized using a response surface central composite design. By regression analysis of the experimental data obtained, the optimum cryoprotectant formulation was found to consist of 14.15 g/100 mL sucrose, 7.07 g/100 mL L-sodium glutamate and 1.01 g/100 mL polyethylene glycol. The predicted survival rate of Candida tropical with the addition of the optimized cryoprotectant formulation after freeze-drying was 81.46%, which was close to the actual value of 82.73%.