荞麦糠皮提取物对Ⅱ型糖尿病大鼠血糖和血脂的影响

Effect of Buckwheat Chaff Extract on Serum Glucose and Lipid Profiles in Type 2 Diabetic Rats

目的:研究荞麦糠皮提取物(BCE)对Ⅱ型糖尿病大鼠血糖和血脂的影响及其机制的探讨。方法:采用链脲佐菌素(STZ)和高脂饲料建立Ⅱ型糖尿病模型。成模大鼠随机分为模型对照组(M组)和荞麦糠皮提取物组(BCE组)。测定空腹血糖(FBG)及用正糖钳实验判断模型是否建立成功。测定各组大鼠血清中甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)及游离脂肪酸(FFA)、谷丙转氨酶(ALT)、谷草转氨酶(AST)的含量。结果:使用STZ和高脂饲料建立的Ⅱ型糖尿病模型成功。BCE能够不同程度地抑制Ⅱ型糖尿病大鼠血清中FBG、FFA、TC、TG、ALT、AST水平的升高,还可明显升高血清中HDL-C水平。结论:BCE可明显影响Ⅱ型糖尿病大鼠血糖和血脂的水平,从而改善胰岛素抵抗。

Objective： To study the effect of buckwheat chaff extract （BCE） on serum glucose and lipid profiles in type 2 diabetic rats and the underlying mechanism. Methods： Type 2 diabetic rat models were established by intraperitoneal injection of streptozotocin （STZ） and feeding high-fat diet and divided into model control group and BCE treatment group. Fasting blood glucose （FBG） and euglycemic clamp technique were used to examine whether type 2 diabetes was successfully induced. All rats were fasted for 12 h after the last administration and blood was taken and assayed for free fatty acids （FFA）, triglyceride （TG）, total cholesterol （TC）, low density lipoprotein cholesterol （LDL-C）, high density lipoprotein cholesterol （HDL-C）, alanine aminotransferase （ALT） and aspartate aminotransferase （AST）. Results： STZ and high-fat diet induced type 2 diabetes in rats. BCE inhibited the increase of FBG, FFA, TC, TG, ALT and AST in the type 2 diabetic rats to different extents but resulted in a significant increase in serum HDL-C level. Conclusion： BCE improves insulin resistance （IR） through affecting serum glucose and serum lipid profiles in type 2 diabetic rats.