乳香-没药配伍前后化学成分溶出变化及其对LPS-诱导的巨噬细胞产生NO的影响

Change in dissolution of chemical components of frankincense-myrrh before and after their compatibility and effect on no release of LPS-induced macrophage cells

目的:分析乳香-没药配伍前后化学成分的变化,评价差异性化合物对LPS-诱导的小鼠腹腔巨噬细胞产生NO的影响,以期从化学成分变化角度探讨乳香-没药配伍协同增效的物质基础。方法:采用UPLC-Q-TOF-MS/MS联用技术对乳香-没药配伍前后化学成分进行分析,并结合MarkerLynx 4.1统计软件分析2味药配伍后的差异性化合物。结果:经PCA分析乳香-没药合提液与合并液色谱图存在明显差异,提示2味药配伍前后化学成分存在显著差异。经OPLS-DA分析及化学成分鉴定,2味药配伍后五环三萜类化合物(α-乳香酸、β-乳香酸等),四环三萜类化合物(榄香酮酸、3-乙酰氧基-16-羟基-24-甲基达玛烷、3-羟基甘遂烷-7,24-二烯-21-酸/3-羟基甘遂烷-8,24-二烯-21-酸等)溶出量显著增加,而环倍半萜类,大环二萜类化合物溶出量均有下降趋势。体外活性评价表明:化合物2-甲氧基-8,12-环氧-吉玛-1(10),7,11-三烯-6-酮,2-甲氧基-5-乙酰氧基-呋喃-吉玛-1(10)-烯-6-酮,3-羰基甘遂-8,24-二烯-21-羧酸显著抑制LPS-诱导的小鼠腹腔巨噬细胞产生NO水平。结论:该研究结果为揭示乳香-没药配伍增强抗炎效应的物质基础提供了一定科学依据与参考。

Objective： To analyze the difference of chemical compounds of frankincense-myrrh before and ,＇after their compati- bility, and evaluate the effect of differentiated compounds on NO generated by LPS-induced peritoneal macrophage cells in rats, in or- der to discuss synergetic material basis of frankincense-myrrh compatibility from the prospective of change in chemleal constituents. Method： UPLC-Q-TOF-MS/MS combined technology was used to analyze the chemical components of frankincense-myrrh before and after their compatibility. MarkerLynx 4. 1 statistical software was used to analyze differentiated compounds before and after their compatibility. Result： The results of PCA showed that there were significant differences in the combined extracts of frankincense- myrrh and the chromatogram of their combined liquid, suggesting significant differences in their chemical compounds before and after their compatibility; after their compatibility, the dissolution of pentacyclic triterpenoid （α-boswellic acid,β-boswellic acid） and tetra- cyclic triterpenoid （elemonic acid, 3-aeetoxy-16-hydroxy-dammar-24-ene, 3-hydroxytirucalla-8,24-dien-21-oic acid or 3-hydroxytiru- calla-7,24-dien-21-oie acid） increased notably, while the dissolution of both yclie sesquiterpenes and macrocyclic diterpenoids de- creased. According to the evaluation on in vitro activity, 2-methoxy-8, 12-epoxy-germa-1 （10） , 7, 11-triene-6-ketone, 2-methoxy-5- acetoxyl-furan-germa-1 （10）-alkene-6-ketone and 3-carbonyl Euphorbia kansui-8, 24-diene-21-earboxylic acid notably inhibited NO generated by LPS-induced peritoneal macrophage cells in rats. Conclusion： These findings provide scientific basis and reference for studies on anti-inflammatory material basis of frankincense-myrrh compatibility.