NF-κB及MIF在育龄期妇女子宫内膜息肉中的表达

Expressions of NF-κB and MIF in endometrial polyps among women of reproductive age

目的:探讨核转录因子κB(NF-κB)、巨噬细胞移动抑制因子(MIF)与育龄期妇女子宫内膜息肉(EPs)发病的关系及其在EPs中表达的相关性。方法:收集宫腔镜手术中42例子宫内膜息肉患者的子宫内膜息肉、息肉周围内膜组织及22例中隔子宫患者正常内膜组织,采用逆转录聚合酶链反应(RT-PCR)技术检测NF-κBp65及MIFmRNA的表达,免疫组织化学S-P法检测NF-κBp65及MIF蛋白的表达。结果:EPs中NF-κBp65及MIF mRNA的表达均高于同期息肉周围内膜及正常子宫内膜(P<0.05),与正常子宫内膜相比,息肉周围内膜中NF-κBp65及MIF mRNA的表达均增强(P<0.05)。NF-κBp65及MIF蛋白在3组中均有表达,均以息肉组中的表达最强,息肉周围内膜组织中的表达次之,3组间两两比较差异有统计学意义(P<0.05)。NF-κBp65及MIF mRNA在EPs中的表达呈正相关(r=0.694,P<0.05),NF-κBp65及MIF蛋白在EPs中的表达呈正相关(r=0.37,P<0.05)。结论:NF-κB和MIF可能与育龄期妇女EPs的发病机制有关,协同参与了EPs发生发展的病理变化。

Objective：To explore the relationship between nuclear factor-kappa B（NF-κB）,macrophage migration inhibitory factor（MIF） and occurrence of endometrial polyps among women of reproductive age and the correlation between NF-κB expression and MIF expression in endometrial polyps.Methods：The samples of endometrial polyps and surrounding endometrial tissue of 42 patients with endometrial polyps and normal endometrial samples of 22 patients with uterus septum were collected after hysteroscopic surgery,RT-PCR technique was used to detect the expressions of NF-κBp65 and MIF mRNA;immunohistochemical SP method was used to detect the expressions of NF-κBp65 and MIF protein.Results：The expression levels of NF-κBp65 and MIF mRNA in endometrial polyps were statistically significantly higher than those in surrounding endometrial tissue and normal endometrium during the same period（P0.05）;compared with normal endometrium,the expression levels of NF-κBp65 and MIF mRNA in surrounding endometrial tissue increased significantly（P0.05）.NF-κBp65 and MIF protein expressed in the three groups,which were the highest in endometrial polyps group,followed by surrounding endometrial tissue,there were statistically significant difference in the expression levels of NF-κBp65 and MIF protein among the three groups（P0.05）.There was a positive correlation between NF-κBp65 expression and MIF mRNA expression in endometrial polyps（γ=0.694,P0.05）,and NF-κBp65 was positively correlated with MIF protein in endometrial polyps（γ=0.37,P0.05）.Conclusion：NF-κB and MIF may be correlated with pathogenesis of endometrial polyps in women of reproductive age,which play synergistic roles in pathological change of occurrence and development of endometrial polyps.