RP-HPLC法同时测定爵床药材中6'-羟基-爵床定B、新爵床脂定B和TaiwanE的含量

RP-HPLC simultaneous determination of 6′-hydroxy-justicidin B, neojusticin B and Taiwan E in Herba Justiciae

目的:建立爵床药材中6'-羟基-爵床定B、新爵床脂定B和Taiwan E 3个活性成分的HPLC含量测定方法。方法:采用Eclipse XDB-C18色谱柱(4.6 mm×150 mm,5μm),流动相为乙腈-水,梯度洗脱(0～9 min,40%乙腈;9～10 min,40%→45%乙腈;10～40 min,45%乙腈),流速0.8 mL·min-1,检测波长256 nm,柱温30℃。结果:6'-羟基-爵床定B、新爵床脂定B和Tai-wan E的线性范围分别为0.023～0.454μg(r=0.9999)、0.022～0.446μg(r=0.9999)和0.024～0.478μg(r=0.9998);平均回收率分别为101.7%(RSD=1.6%)、95.7%(RSD=0.30%)和99.4%(RSD=1.7%)。结论:本方法可用于爵床药材的质量控制。

Objective ：To develop a RP - HPLC method for the determination of three active components,6＇ - hy- droxy -justicidin B,neojusticin B and Taiwan E,in Herba Justiciae. Methods：The sample was analyzed on a E- clipse XDB - C18 column （4.6 mm× 150 mm, 5μm ） with a mobile phase consisting of aeetonitrile and water （0 - 9 rain,40% acetonitrile ;9 - 10 rain,40% -45% acetonitrile ; 10 - 40 rain,45% acetonitrile） at the flow rate of 0. 8 mL . min - 1. The column temperature was set up at 30 ℃, and the detection wavelength was at 256 nm. Results： The linear ranges of 6＇ - hydroxy - justicidin B, neojusticin B and Taiwan E were 0. 023 - 0. 454μg（ r = 0. 9999 ） , 0. 022 - 0. 446 μg（ r = 0. 9999 ） and 0. 024 - 0. 478μg （ r = 0. 9998 ）, respectively. The average recoveries of the three components were 101.7% （ RSD = 1.6% ） ,95.7% （ RSD =0. 3% ） and 99.4% （ RSD = 1.7% ） ,respectively. Conclusion：The method can be used for the quality control of Herba Justiciae.