硫磺熏蒸前后白芍HPLC-UV特征图谱的比较研究

Comparative study on HPLC-UV specific chromatograms of Paeoniae Radix Alba before and after sulfur-fumigation

目的:对白芍硫磺熏蒸前后的HPLC-UV特征图谱进行比较研究,为科学评价及有效控制其质量提供可靠方法。方法:采用RP-HPLC方法,使用Hypersil ODS C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-0.5%醋酸水溶液,流速为1.0 mL·min-1,二元梯度洗脱,二极管阵列检测器,测定未经硫磺熏蒸和经硫磺熏蒸的白芍各10批,以芍药苷为参照物,检测波长为270 nm。结果:未经硫磺熏蒸的白芍中共有23个共有峰,经硫磺熏蒸的白芍中共有18个共有峰,通过与对照品的保留时间及紫外光谱比较,5、13、16、17号峰的归属分别为没食子酸、儿茶素、白芍苷、芍药苷。结论:白芍经硫磺熏蒸后,其成分发生了比较明显的变化,同时白芍在硫磺熏蒸前后,某些共有成分的含量也有不同程度的改变,硫磺熏蒸确实对白芍的质量有非常大的影响。

Objective ： To comparatively analyze HPLC - UV specific chromatograms of Paeoniae Radix Alba before and after sulfur -fumigation, so as to provide reliable methods for scientifically evaluating and effectively controlling the quality of Paeoniae Radix Alba. Methods： RP - HPLC method was employed, and the chromatographic condi- tions were as follows ： Hypersil ODS C18 column （250 mm × 4.6 mm,5μm） , binary solvent delivery gradient elution of acetonitrile -water（ containing 0.5% acetic acid） as mobile phase with a flow rate of 1.0 mL . min-1, photodi- ode array detector. The detective wavelength was set at 270 nm, and ten batches of sun - dried and sulfur - fumiga- ted Paeoniae Radix Alba were respectively analyzed using paeoniflorin as the reference compound. Results：A satis- factory method for HPLC - UV specific chromatograms of Paeouiae Radix Alba before and after sulfur - fumigation was established. Totally 23 common peaks were observed in the specific ehromatogram of sun - dried Paeoniae Radix Alba ,while only 18 common peaks were found in the specific chromatogram of sulfur- fumigated Paeoniae Radix