GC法同时测定明目上清片中4个成分的含量

GC simultaneous determination of four ingredients in Mingmushangqing tablets

目的:建立同时分析测定明目上清片中4个活性成分(柠檬烯、薄荷酮、薄荷脑和胡薄荷酮)的方法。方法:采用GC法,色谱柱为DB-1701毛细管色谱柱(30 m×0.3 mm,0.25μm),柱温采用程序升温(80℃保持1 min,5℃.min-1上升至120℃,保持1 min,再以50℃.min-1的速率升温至220℃,保持2 min);进样口温度:180℃;检测器:FID;检测器温度:250℃。结果:柠檬烯、薄荷酮、薄荷脑和胡薄荷酮线性范围分别为0.0103～0.507 mg·mL-1(r=0.9997)、0.0105～0.501 mg·mL-1(r=0.9999)、0.0082～0.412 mg·mL-1(r=0.9991)、0.0085～0.404 mg·mL-1(r=0.9998);平均加样回收率(n=6)分别为99.1%、97.6%、98.7%、98.3%,RSD分别为1.2%、1.8%、1.4%、1.5%。结论:该方法可用于明目上清片的质量检测。

Objective：To establish a method for simultaneous determination of four active ingredients （limonene, menthone, menthol, pulegone） in Mingmushangqing tablets. Methods：The determination was performed on a DB - 1701 column（30m×0.3mm,0.25μm）. The column temperature was maintained at 80 ℃ for 1 min,and pro- grammed to 120 ℃ at the rate of 5 ℃ . min-1 ,then held for 1 min. After that the temperature was elevated to 220 ℃ at the rate of 50 ℃ . min-1. The FID was used as detector, the temperatures of the injection and the detector were set at 180 ℃ and 250 ℃ and maintained for 2 min. Results： The calibration curves were linear in the ranges of 0. 0103 -0. 507 mg . mL-1 for limonene （r = 0. 9997） ,0. 0105 -0. 501 mg. mL-1 for menthone （r = 0. 9999 ） , 0. 0082 - 0. 412 mg. mL- 1 for menthol （ r = 0. 9991 ）, and 0, 0085 - 0, 404 mg . mL- 1 for pulegone （ r = 0. 9998 ）. The average recoveries （n = 6） were 99. 1% ,97.6% ,98.7% and 98.3% ;RSDs were 1.2% , 1.8% , 1.4% and 1.5 % , respectively. Conclusion： The method can be used for quality control of Mingmushangqing tablets.