摘要
为了提高汽爆水稻秸秆还原糖的转化率,采用纤维素酶对水稻秸秆进行酶解实验。还原糖的含量用3,5-二硝基水杨酸法进行测定。实验选用加酶量、酶解时长和反应温度作为考察因素,以原始水稻秸秆与蒸汽爆破预处理水稻秸秆作对比。结果表明纤维素酶用量占秸秆干物质质量的10%,酶解时长48 h,反应温度50℃是一个较为理想的反应条件;原始水稻秸秆最大酶解还原糖产量约为9.7%;蒸汽爆破水稻秸秆最大酶解还原糖产量约为34.3%;蒸汽爆破预处理能够显著提高水稻秸秆的酶解还原糖产量,并缩短酶解反应时间。
For the purpose to improve the conversion rate of reducing sugar of rice straw,the cellulose was used in the enzymatic hydrolysis of rice straw. The content of reducing sugar was detected by 3,5-dinitrosalicylic acid method. The enzyme concentration, hydrolysis time and temperature were selected as inspecting factors. Meantime, comparisons of the enzymatic hydrolysis of rural rice straw with that of steam explosion pretreatment rice straw were made. The results showed when the cellulose weight content taking 10% of the dry rice straw, enzymatic hydrolysis time of 48 h and reaction temperature of 50℃ were ideal reaction conditions. The maximum reducing sugar yield of enzymatic hydrolysis original rice straw was 9.7%. The highest reducing sugar yield of enzymatic hydrolysis steam explosion rice straw was approximately 34.3%. Steam explosion pretreatment can obviously improve the production of reducing sugar of enzymatic hydrolysis rice straw, and at the same time accelerate the reaction rate.
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2013年第1期106-112,共7页
Transactions of the Chinese Society for Agricultural Machinery
基金
江苏省科技支撑计划资助项目(BE2010356)
江苏省农机三项工程资助项目(NJ2010-42)
关键词
水稻秸秆
蒸汽爆破
纤维素
酶解
还原糖
3
5-二硝基水杨酸法
Rice straw
Steam explosion
Cellulose
Enzymatic hydrolysis
Reducing sugar3,5-dinitrosalicylic acid