七氟醚后处理对大鼠海马脑片氧糖剥夺损伤的保护作用

Protective effects of sevoflurane postconditioning on oxygen-glucose deprivation injury in rat hippocampal slices

目的观察七氟醚后处理对大鼠海马脑片氧糖剥夺损伤(OGD)的保护作用。方法将符合标准的40片海马脑片随机均分为四组:2%七氟醚后处理组(2%Sev组)、4%七氟醚后处理组(4%Sev组)、6%七氟醚后处理组(6%Sev组)和氧糖剥夺损伤对照组(OGD组)。采用脑片灌注及电生理技术细胞外记录海马CA1区的顺向群锋电位(OPS)。利用2,3,5-三苯基氯化四氮唑(TTC)染色定量比色方法分析脑片损伤程度。结果 OGD组OPS恢复程度为(3.14±2.56)%,恢复率为10%,组织损伤率为0.63±0.05;2%Sev组OPS恢复程度为(46.24±29.34)%,OPS恢复率为40%,组织损伤率为0.51±0.06;4%Sev组OPS恢复程度为(62.40±34.93)%,OPS恢复率为60%,组织损伤率为0.41±0.07;6%Sev组OPS恢复程度为(75.92±45.31)%,OPS恢复率为70%,组织损伤率为0.37±0.06。与OGD组比较,2%Sev组、4%Sev组、6%Sev组的OPS恢复程度和OPS恢复率均明显升高,组织损伤率明显降低(P<0.01)。结论七氟醚后处理对大鼠海马脑片氧糖剥夺损伤具有一定的保护作用。

Objective To investigate the protective effects of sevoflurane postconditioning on oxygen-glucose deprivation injury in rat hippocampal slices. Methods Forty hippocampal slices were randomly divided into 4 groups （n= 10 each） ： group OGD; group 2% Sev; group 4% Sev; group 6% Sev. The technique of electrophysiology was used, and the amplitude of orthodromic population spike （OPS） in the stratum pyramidale of the CA1 region was measured. And TTC staining was used to calculate the percentage of tissue injury. Results OPS recovery amplitude was （3.14±2.56） %, OPS recovery rate was 10%, and the degree of tissue injury was 0.63±0.05 in group OCJD. OPS recovery amplitude was （46.24±29.34）%, OPS recovery rate was 40%, and the degree of tissue injury was 0.51±0. 06 in group 2% Sev. OPS recovery amplitude was （62.40±34.93）%,OPS recovery rate was 60%, and the degree of tissue injury was 0. 41±0.07 in group 4% Sev. OPS recovery amplitude was （75.92±45.31）% ,OPS recovery rate was 70%, and the degree of tissue injury was 0.37±0. 06 in group 6% Sev. The degree and rate of recovery of OPS were increased but the tissue injury was deceased in group 2% Sev, group 4% Sev and group 6% Sev as compared with OGD group. Conclusion Sevoflurane postconditioning can protect neurons from oxygen-glucose deprivation injury in rat hippocampal slices.