摘要
BCR-ABL融合基因可编码产生BCR-ABL融合蛋白,是慢性粒细胞白血病的特征性标志,对慢性粒细胞白血病的诊断、治疗具有重要意义。目前临床有多种方法可对其进行检测,主要有细胞遗传学、荧光原位杂交、聚合酶链式反应、Western Blot印迹法、流式免疫磁珠(CBA)等检测方法。这些检测方法有的费时费力,有的需要专门的实验设备等,故在临床应用中受限。随着对BCR-ABL融合基因检测技术的发展,CBA法对BCR-ABL融合蛋白的检测具有其特有的优越性而得到越来越多的应用。
BCR-ABL fusion gene can produce BCR-ABL fusion protein by coding,which is the charac- teristic sign of chronic myelogenous leukemia（CML） and is of great significance in the diagnosis and treat- ment of CML. Currently there are many clinical testing methods, such as conventional chromosome detection （CC） , fluorescent in situ hybridization （FISH） , palymerase chain reaction （PCR） , Western blot （WB） , flow immunomagnetic beads（CBA） and so on. Some of the methods axe relatively time-consuming and laborious and some need special experimental equipments, therefore axe limited in the clinical application. With the de- velopment of detection technology of BCR-ABL fusion gene, the CBA method to detect BCR-ABL fusion pro- tein are applied increasingly for its unique superiority.
出处
《医学综述》
2013年第1期137-141,共5页
Medical Recapitulate
