广西汉族TUBB3基因表达与非小细胞肺癌关联性的研究

Correlation analysis of TUBB3 expression and non-small cell lung cancer in patients of Han Nationality in Guangxi province

目的探索广西地区非小细胞肺癌(NSCLC)汉族患者中,Ⅲ类β-微管蛋白(TUBB3)基因mRNA的表达及其临床预后意义。方法采用逆转录-聚合酶链反应(RT-PCR)方法检测60例肿瘤组织以及其中34例癌旁组织中TUBB3基因mRNA的表达。结果 TUBB3在癌及癌旁组织中的表达分别为16.7%和2.9%(P=0.053),TUBB3表达时,淋巴结转移高于无淋巴结转移(80.0%和13.5%,P=0.014)。其余临床特征未发现任何有统计学意义的相关性。结论 TUBB3mRNA在汉族非小细胞肺癌患者中阳性表达可能代表一个晚期事件。

Objective To investigate the expression of class 1][ ^-tubulin （TUBB3） and its clinical sig- nificance in patients of Han nationality with non-small cell lung cancer （NSCLC） in Guangxi province. Methods The mRNA expression of TUBB3 gene was examined in 60 tumor tissues and 34 adjacent tissue samples using semi-quantitative RT-PCR. Results The expression rate ofTUBB3 mRNA was 16.7% in tumor tissues and 2.9% in its adjacent tissues （P=0.053）, which was higher in the tissues with metastasis of lymph nodes （80.0%） than that without metastasis of lymph nodes （13.5%）, P=0.014. No statistical correlation between TUBB3 expression and other clinical features were found. Conclusion Positive expression of TUBB3 mRNA in the Hart nationality might indicate an advanced stage event.