蓝舌病病毒VP7蛋白的B细胞表位预测

PREDICTION OF B CELL EPITOPES IN MAJOR CORE PROTEIN VP7 OF BLUETONGUE VIRUS

根据前期试验中测得的蓝舌病病毒VP7蛋白的基因序列和推导的氨基酸序列，利用DNAStar软件和Biosun软件进行生物信息学预测，以单参数（亲水性、可及性、柔韧性、抗原性）预测为基础，结合二级结构预测来综合分析蓝舌病病毒VP7蛋白的B细胞表位。比较两种软件的预测结果发现，在蓝舌病病毒VP7蛋白的381个氨基酸序列中，第81～85、198～202、235～239、253～257区域有较好的亲水性、表面可及性和较高的抗原指数，并且在二级结构上含有易形成抗原袁位的转角和无规则卷曲，最有可能为蓝舌病病毒VP7蛋白的B细胞线性优势表位。上述预测和判定结果表明，在蓝舌病病毒VP7蛋白序列中存在优势B细胞线型表位，为进一步合成多肽并分析已获得的VP7蛋白单抗的结合表位奠定了基础。

The nucleotide and deduced amino acid sequences ofBTV VP7 protein were obtained from our laboratory. The B cell epitopes of the BTV VP7 protein were predicted by using DNAStar software and Biosun software to evaluate single parameters （hydrophilicity, accessibility, flexility and antigenicity） in combination with the secondary structure. In the 350 aa region of BTV VP7 protein, we identified five epitopes locating in 81-85, 198-202, 235-239, 253-257 aa, which could be larvaceous dominant B-cell linear epitopes. This prediction provided a theoretical basis for identification of monoclonal antibodies against these epitopes on major core protein VP7 of Bluetongue virus.