石油污染地下水中苯降解菌的代谢特征

Metabolic Characteristics of Benzene by Strain from Petroleum-Contaminated Groundwater

从东北某油区石油污染的地下水中分离、纯化出苯降解优势菌B6,研究其降解效率和代谢特征.结果表明:当模拟石油污染地下水中苯的质量浓度为394.36mg/L时,3d后的降解率为82.82%;相关酶活及代谢产物在双加氧酶的作用下,B6菌降解苯的途径为儿茶酚正位裂解;B6菌在代谢过程中产生色氨酸酶、脂肪酶、淀粉酶、明胶酶、乳糖酶和氧化酶等,葡萄糖、乳糖、蛋白质、色氨酸、脂肪、淀粉、明胶、多肽、纤维素、含硫有机物和细胞色素C等为其营养物质,以亚硝酸盐和硝酸盐为电子受体;B6菌为革兰氏阳性红平红球菌.

The authors investigated the degradation features of benzene by strain B6 dominant strain isolated and purified from the petroleum-contaminated groundwater in the northeast of China. Results show that when the initial mass concentration of benzene was 394.36 mg/L, the degradation rate of benzene by train B5 was 82.82% on the third day ortho 2 cleavage of catechol was the right approach of benzene degradation under the effect of dioxygenase B6 strain could produce tryptophanase, lipase, amylases, gelatinase, lactase, oxidase, use glucose, lactose, protein, tryptophan, fat, starch, gelatine, polypeptide, cellulose, sulfur-containing organic compounds, cytochrome C as nutrients, and utilize nitrites and nitrates as electron acceptors in its metabolic process. It was identified that B6 strain was Rhodococcus erythropolis, gram-positive bacterium.