采用ELISA差减分析法探讨武汉地区中老年患者IEM HBsAg的携带状态

The investigation of immune escape mutant HBsAg in the quinquagenarian carriers in Wuhan area by ELISA differential analysis

目的探讨武汉地区中老年患者血清免疫逃逸变异表面抗原(IEM HBsAg)携带状态。方法以2种不同抗HBsmAb包被,采用酶联免疫吸附法(ELISA)对1641例中老年既往HBV感染者的血清标本进行检测,并以中和实验及广谱IEMHBsAg试剂对单一阳性者血清HBsAg检测的特异度进行验证。结果所建立的G6-ELISA与E2-ELISA具有大致相同的稳定性、特异度与灵敏度,对该组血清检测的阳性率分别为29.37%、29.01%。6例G6-ELISA单一阳性血清中,抗HBs、抗HBe、抗HBc、HBeAg及HBV DNA阳性例数分别为3、1、6、1、2例,抗HBs中和实验阳性率为100.00%,平均中和率81.14%。雅培化学发光试剂复核此6例均为阳性结果。结论结合文献与该课题组既往研究分析,该组中老年患者中存在少量呈优势表达的IEMHBV感染者。

Objective To investigate the situation of the immune escape mutant HBsAg in the quinquagenarian carriers in Wuhan area by using ELISA differential analysis.Methods Two different anti-HBs mAbs were used as coated antibodies to detect HBsAg in 1 641 quinquagenarian HBV carriers under the same reagents and operation condition.Neutralization reactions and broad spectrum immune escape mutant HBsAg reagents were used to verify the specificity of the HBsAg positive reaction in single regent above-mentioned.Results The two reagents respectively named G6-ELISA and E2-ELISA showed similar in the stability,specificity and sensitivity.The positive rates of the two reagents in detecting the quinquagenarian serum HBV carriers were 29.37% and 29.01%,respectively.In the 6 positive samples which were detected by single G6-ELISA,the positive samples of anti-HBs,anti-HBe,anti-HBc,HBeAg and HBV DNA were 3,1,6,1 and 2 respectively.The positive rate in anti-HBs neutralization test was 100.00%,and mean neutralization rate was 81.14%.6 samples all showed positive reactions in Abbott chemoluminescence regent.Conclusion Combining the bibliographical information and former researches,there were few carriers whose HBV immune escape mutant HBsAg express as dominant strain in serum in this group of quinquagenarian HBV carriers.