温和灸对慢性疲劳大鼠肠道菌群的影响

Influence of Mild Moxibustion on Intestinal Microflora of Chronic Fatigue Rats

目的观察温和灸对慢性疲劳大鼠肠道菌群的影响,并探讨其机理。方法 40只大鼠随机分为正常组、模型组、人参皂苷组、温和灸组。除正常组外,其他大鼠用饮食失节加冷水游泳制备慢性疲劳模型。正常组和模型组不给予任何治疗,温和灸组灸关元,人参皂苷组给予人参皂苷水溶液灌胃,每日1次。21 d后,被迫采取粪便,采用厌氧及需氧菌选择性培养基测定各组大鼠粪便中典型菌群数量。结果与正常组比较,模型组菌群数量明显减少(P<0.05);与模型组比较,温和灸组和人参皂苷组菌群数量明显增加(P<0.05);温和灸组与人参皂苷组比较差异无统计学意义(P>0.05)。结论关元灸可有效升高肠道中双歧杆菌、乳酸杆菌2种厌氧菌及大肠杆菌、肠球菌2种需氧菌的菌群数量,调整肠道菌群失调。

Objective To study influence of mild moxibustion on intestinal microflora of chronic fatigue rats,and explore its mechanism.Methods Forty rats were randomly divided into normal group,model group,ginsenoside group and mild moxibustion group.Rat model of chronic fatigue was made by disorder diet and cold-water swimming.Normal group and model group were not given any treatment,mild moxibustion group was given moxibustion at Guanyuan point,ginsenoside group was intragastrically given ginsenoside solution,once a day.After 21 days,forced to take dung,anaerobic and aerobic bacteria were cultured with selective media and determined the number of typical flora.Results Compared with the normal group,the number of bacteria of model group reduced significantly（P〈0.05）.Compared with model group,bacteria of mild moxibustion group and ginsenoside group increased significantly（P〈0.05）,and there was no significant difference between mild moxibustion group and ginsenoside group（P〈0.05）.Conclusion Guanyuan moxibustion can improve symptoms of rats with diarrhea,adjust the intestinal flora： increase the number of two kinds of anaerobic bacteria（bifidobacteria,lactobacilli） and two kinds of aerobic bacteria（enterobacter,intestinal bacteria）.