百里醌对胆管结扎大鼠氧化应激和肝损伤的保护作用研究

Protection of Thymoquinone Against Oxidative Stress and Hepatic Injury After Biliary Ligation in Rats

目的研究百里醌对总胆管结扎大鼠氧化应激和肝损伤的保护和治疗作用。方法 32只SD大鼠随机分为4组,分别为正常对照组、模型对照组、百里醌低剂量和高剂量组,每组8只。在胆管结扎术前3 d起灌胃给予百里醌,低剂量组为25 mg.kg-1,高剂量组为50 mg.kg-1,连续2周。处死大鼠,检测肝组织匀浆中羟脯氨酸(HP)含量和丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPx)的活性。苏木精-伊红(HE)染色检测肝组织病理学改变。结果百里醌可显著降低胆总管结扎引起的肝脏组织内HP和MDA的含量,升高SOD和GPx含量(P<0.05)。百里醌治疗组肝脏坏死面积较模型对照组显著降低,炎性浸润程度明显降低。结论百里醌可以提高抗氧化损伤能力,减少肝脏氧化应激破坏,有望在胆汁淤积症患者中用于肝功能的保护。

Objective The aim of this study was to investigate the preventive and therapeutic effect of thymoquinone against oxidative stress and hepatic injury after biliary ligation in rats. Methods A total of 32 Sprague - Dawley rats were randomly divided into 4 groups： normal control, model control, low-dose and high-dose thymoquinone groups. Each group contained 8 animals. The rats in thymoquinone-treated groups were given with thymoquinone （25 or 50 mg . kg-1 body weight） orally for 2 weeks starting 3 days prior to bile duct ligation. The content of hydroxyproline （ HP）, malondialdehyde （ MDA）, superoxide dismutase （SOD） and glutathione peroxidase（GPx） in hepatic tissues was determined. Histopathological examination of liver was carried out. Results Thymoquinone treatment significantly decreased the elevated HP content, and MDA levels and raised the lowed level of SOD, and GPx enzymes in the tissues （P 〈 0. 05 ）. Thymoquinone attenuated necrosis and inflammatory infiltration in the livers of rats with bile duct ligation. Conclusion The present study demonstrates that oral administration of thymoquinone in bile duct ligated rats maintains antioxidant defenses and reduces liver oxidative damage of body, which may be useful in pretecting liver function of cholestasis.