野菊花中的黄酮类化学成分

Isolation and Identification of Flavonoids from Flos Chrysanthemi Indici

目的研究野菊花乙醇提取物中乙酸乙酯层化学成分。方法采用硅胶柱色谱、ODS柱色谱、SephadexLH-20柱色谱和制备液相色谱等手段进行分离、并根据理化性质和光谱数据鉴定化合物结构。结果从野菊花中分离得到10种黄酮类化合物,分别鉴定为5,7,4'-三羟基-3',5'-二甲氧基黄酮(1)、5,7,3',4'-四羟基-6,5'-二甲氧基黄酮(2)、槲皮素(3)、木犀草素(4)、槲皮素-7-O-β-D-葡萄糖(5)、刺槐素-7-O-β-D-葡萄糖(6)、香叶木素-7-O-β-D-葡萄糖苷(7)、木犀草素-7-O-β-D-葡萄糖苷(8)、芹菜素-7-O-β-D-葡萄糖苷(9)、蒙花苷(10)。结论槲皮素-7-O-β-D-葡萄糖首次从野菊花中分离得到。

Objective To investigate the chemical constituents in the EtOAe fraction of ethanol extracts from Flos Chrysanthemi Indici. Methods The compounds were isolated by silica gel chromatography, ODS chromatography, Sephadex LH-20 chromatography, and preparative HPLC, and their structures were elucidated on the basis of chemical and spectroscopic methods. Results Ten flavonoids were isolated from Flos Chrysanthemi Indici, and were identified as triein（ 1 ）, 5, 7, 3＇, 4＇- tetrahydroxy-6, 5 ＇-dimethexy-flavone （ 2 ）, quereetin （ 3 ）, luteolin （ 4 ）, queertin-7-O-β-D-glucopyranoside （ 5 ）, acacetin7-O-β-D- glueopyranoside （ 6 ）, diosmetin-7-O-β-D-glueopyranoside （ 7 ）, luteolin-7-O-β-D-glucopyranoside （ 8 ）, apigenin-7-O-β-D- g｜ueopyranoside（9）, and linafin （10）. Conclusion Qucertin 7-O-]3-D-glueopyranoside is isolated from Flos Chrysanthemi Indiei for the first time.