二阶段化学诱发小鼠肝癌模型C-MYC基因动态变化的研究

The Dynamic Change of C-MYC Gene in Two-Stage Chemical Induced Liver Cancer Model in Mice

目的探讨癌基因C-MYC在化学诱导小鼠肝癌过程中的动态变化。方法取C57BL/6J雄性健康小鼠60只(实验组),二甲基亚硝胺(DEN)/四氯化碳(CCl4)/乙醇联合诱导20周;取60只同种小鼠为对照组。每2周随机抽取小鼠处死,取肝组织进行病理学观察,并采用实时荧光定量PCR检测诱癌组和相应时期对照组中C-MYC基因表达变化情况。结果 DEN/CCl4/乙醇诱导20周后,成功诱发小鼠肝癌。实时荧光定量PCR结果显示,C-MYC mRNA在第4周和第6周实验组和对照组差异无统计学意义(P>0.05),从第8周开始C-MYC mRNA的表达量逐渐升高,以诱癌第18～20周其表达升高尤为显著,实验组明显高于对照组(P<0.05)。结论在肝癌的诱癌过程中,C-MYC基因表达量随着诱癌的时间延长而逐渐增加,在肝癌阶段异常高表达,C-MYC基因可能促进肝癌的发生、发展。

Objective To investigate the dynamic change of C-MYC oncogene in the process of chemical induced hver cancer in mice. Methods Sixty healthy C57BL/6J male mice were induced to establish the hepatocellular carcinoma （HCC） model by combination of diethylnitrosamines （DEN）, carbon tetrachloride （CC14） and ethanol for twenty weeks（ experimental group） ,and another sixty C57BL/6J mice as normal controls. The mice were randomly put to death every two weeks and observed the pathology of their liver tissues. Real-time fluorescent quantitative PCR was used to detect the change of expression of C-MYC gene in experimental group and control group.Results After 20-week DEN/CC14/ethanol induction,HCC model of mice were successful induced. The result of real-time fluorescent quantitative PCR suggested that no significant difference was found in C-MYC mRNA between experimental group and control group of the fourth week and the sixth week（ P 〉 0.05 ）. C-MYC expression quantity was gradually increased from the eighth week, and the increasing was most significant during the eighteenth week and the twentieth week, which was higher in the experimental group than the controls （P 〈 0.05 ）. Conclusion In the process of induced HCC model in mice, C-MYC gene expression quantity is gradually increased with cancer-induced time prolongs, C-MYC gene shows abnormal high expression in HCC stage and may promote the occurrence and development of HCC.