鲁米诺-高锰酸钾化学发光法测定芦丁片中芦丁的含量

Content Determination of Rutin in Rutin Tablets by Luminol-potassium Hypermanganate Chemiluminescence Method

目的:建立一种快速、灵敏地测定芦丁含量的新方法。方法:利用在碱性条件下,芦丁对鲁米诺-高锰酸钾体系有强烈的增强作用的原理,结合流动注射技术,建立了流动注射化学发光法检测芦丁的新方法;反应试剂的浓度分别为:鲁米诺(2.0×10-5mol/L)、高锰酸钾(8.0×10-5mol/L)、氢氧化钠溶液(0.05mol/L),主泵(P1)、副泵(P2)流速分别为2.12、3.45ml/min,光电倍增管负高压为600V,采样时间为15s。结果:芦丁的检测质量浓度线性范围为2.0×10-9～2.0×10-7g/m(lr=0.9991),检出限为8.3×10-10g/ml,平均加样回收率为101.57%,RSD=1.69%(n=3)。结论:该方法简单、快速、灵敏性高,为芦丁药物的质量控制和体内分析提供了一种新的检测方法。

OBJECTIVE： To establish a novel, rapid and sensitive method for the determination of rutin. METHODS： Rutin was found to enhance the chemiluminescence （CL） signal of luminol-potassium hypermanganate system in alkaline medium. Based on flow injection （FI）, a new FI-CL method was developed for the determination of rutin. The concentrations of reagents were lu- minol （2.0×10^- 5mol/L）, potassium hypermanganate （8.0×10^-5 mol/L）, sodium hydrate （0.05 mol/L） ; the flow rates of pumps were set at 2.12 ml/min （P1） and 3.45 ml/min （P2） with negative high voltage of 600 V and sampling time of 15 s. RESULTS： The linear range of rutin was 2.0×10^-9-2.0×10^-7 g/ml （r=0.999 1）with average recovery of 101.57% （RSD=1.69,n=3）. The detec- tion limit was 8.3×10^-10 g/ml. CONCLUSIONS： The method is convenient, rapid and sensitive, and can provide a new method for the quality control and product detection of rutin.