乌司他丁对小鼠全氟异丁烯吸入性肺损伤的作用

Effects of ulinastatin on acute lung injury induced by perfluoroisobutylene inhalation in mice

目的观察乌司他丁对全氟异丁烯吸入性急性肺损伤的防治效果。方法 采用小鼠全氟异丁烯全身暴露动态染毒系统对实验小鼠进行染毒,观察不同剂量的乌司他丁于全氟异丁烯暴露后不同时间尾静脉注射给药的存活率、肺系数(湿肺体比、干肺体比、肺含水量、肺湿干比)。结果在全氟异丁烯暴露后不同时间(1、2、4、8h)经尾静脉注射给药不同剂量乌司他丁(40万、80万U/kg),除于暴露后4和8h乌司他丁40万U/kg组观察到湿肺体比和肺湿干比给药组较对照组明显下降(P均<0.05)外,不论是肺系数还是中毒小鼠存活率观察,均未观察到乌司他丁对全氟异丁烯染毒小鼠明显的治疗作用(P均>0.05)。结论在本项研究的实验条件下,未观察到乌司他丁对全氟异丁烯吸入性急性肺损伤具有治疗作用。

Objective To observe the prophylactic and therapeutic efficacy of ulinastatin on acute lung injury induced by perfluoroisobutylene（PFIB）inhalation in mice.Method The PFIB -toxicant exposure model of mice was established using the method exposed to gaseous PFIB in a flow-past whole-body exposure system.After model established,ulinastatin of different doses（4×105 or 8×105U/kg）or equal volume water（control group）was administrated by caudal vein to mice.Then the mice were killed,and the lung was separated.The survival rate and lung coefficient（including wet lung-to-body weight ratio,dry lung-to-body weight ratio,lung water content and lung wet-to-dry weight ratio）were calculated to observe the therapeutic effect of ulinastatin at different time and different dose on PFIB-toxicant exposure mice.Results The therapeutic effects of ulinastatin neither on survival rate nor on lung coefficient were observed（all P0.05）,except the wet lung-to-body weight ratio and the lung wet-to-dry weight ratio in 4×105U /kg ulinastatin administration group at 4 h and 8 h post PFIB exposure（all P0.05）.Conclusion The result reported in this paper shows that no prophylactic or therapeutic effects of ulinastatin on ALI induced by PFIB inhalation were observed,but further study is still neccesary.