摘要
目的:建立柱前衍生化HPLC法测定加巴喷丁胶囊中的主药含量。方法:对加巴喷丁柱前衍生化后进行测定,选择Sinochrom-ODS(250 mm×4.6 mm,5μm)为固定相,0.33 mol.L-1乙酸水溶液-甲醇-乙腈(30∶70∶30)为流动相等度洗脱,流速1.0 mL.min-1,检测波长336 nm。结果:加巴喷丁在1.0～12.0 mg.L-1质量浓度范围内线性关系良好,其平均加样回收率为99.4%(n=9),RSD为0.84%;测得样品中加巴喷丁含量为标示量的99.82%。结论:该法便捷可靠,专属性强,可用于加巴喷丁胶囊中主药的含量测定。
Objective： To establish a pre-column derivation HPLC method for content determination of 0. 33 mol. L - 1 acetic acid solution-methanol-acetonitrile （30： 70： 30） was used as mobile phase for isocratic elution, with the flow rate of 1.5 mL.min-t. The detection wavelength was 336 nm. Results： A good linear relationship was shown in the gabapentin concentration range of 1.0 ~ 12. 0 mg-L-1. The average recovery was 99.4%, with RSD of 0. 84% （ n = 9）. The average content of gabapentin in gabape accounted for 99. 8% of the labeled amount. Conclusion： The method is specific, convenient It can be used for the determination of the main component in the gabapentin capsules. ntin capsules and reliable.
出处
《药学进展》
CAS
2013年第1期38-42,共5页
Progress in Pharmaceutical Sciences
