BALB/C小鼠不同组织RNA提取方法的比较

Comparison of RNA extraction method from different tissues of BALB/C mouse

为获得高质量BALB/C小鼠的核酸用于荧光定量RT-PCR分析,用某公司的RNA试剂盒、TRIzol试剂、氯化锂分别提取BALB/C小鼠心脏、肝脏和肾脏组织中的RNA。经紫外分光光度计检测发现,用TRIzol试剂在小鼠肾脏组织中获得的核酸片段具有更高的纯度及浓度,而1%琼脂糖凝胶电泳检测发现三种来源的RNA均具有较好的完整性。RT-PCR方法扩增小鼠主要组织相容性复合体蛋白H-2K编码基因,3个样品均可扩增出104 bp的特异性条带。试验验证了TRIzol试剂从不同组织中提取小鼠总RNA均可作为RT-PCR的模板,为后续试验工作奠定基础。

In order to obtain high quality nucleic acid of BALB/C mice used for the fluorescence quantitative RT-PCR analysis, RNA kit of some Company, TRIzol Reagent and chlorine lithium were used to extract total RNA from heart, liver and kidney. Total RNA obtained from kidney by TRIzol reagent showed higher purity and concentration by UV spectrophometer. All total RNA in this study showed good integrity determined by 1% agarose gel electrophoresis analysis. Specific bands of 104 bp fragment encoding mouse major histocompatibility complex protein H-2K gene were amplified by RT-PCR from three total RNA. The experiment identified that total RNA could be extracted from various tissues of mouse by TRIzol and used as RT-PCR template. It laid a foundation for the subsequent experimental work.