在猪组织笼内恩诺沙星对大肠杆菌的药效研究

Pharmacokinetic effects of enrofloxacin against Escherichia coli in tis sue cage of pigs

为了合理应用恩诺沙星治疗猪大肠杆菌感染,采用猪体内安装组织笼的方法,研究感染组织笼用药后,组织笼内大肠杆菌数量的变化。结果表明,恩诺沙星在不同大小的组织笼内的药动学特征不同,在小组织笼内的半衰期明显大于在大组织笼内的半衰期,在相同大小的组织笼内随着药物浓度的增加半衰期表现为增长。感染笼注射恩诺沙星前后细菌的生长繁殖情况亦有不同,在大组织笼和小组织笼内,无药对照笼内的细菌在试验的过程中细菌的数量均基本上保持恒定,大组织笼内注入2MIC的恩诺沙星后,细菌在被抑制9 h后,出现再生长现象,而在小组织笼内细菌在被抑制12 h后,出现再生长现象。注入5MIC或8MIC的恩诺沙星后,大小组织笼内的5MIC和8MIC的细菌杀菌曲线几乎重合,并都在24 h后,在组织笼内检测不到细菌。细菌在接触药物的早期阶段下降最为迅速。通过分析认为,如果药物在体内消除慢,可以适当减少抗菌药物的用量。

For researching the usage of enrofloxacin against Escherichia coil, the method of tissue cage was used to survey the amount of Escherichia coil in tissue cage after administration. The results showed that on the pharmacokinetic characters of enrofloxacin was between different big and small tissue cages. The half-life of enrofloxacin in small tissue cage was more than that in big one. The half-life in the same tissue cage would increase as the concentration increased of enrofloxacin. The growing character of Escherichia coli in tissue cage was different before and after administration. The amount of Escherichia coli in control tissue cage was almost stable. Escherichia coli was inhibited for 9 h and regrowed in big tissue cage, and was inhibited for 12 h in small one after 2MIC enrofloxacin injected. The killing curve of 5MIC and 8MIC of enrofloxacin against Escherichia coli was almost coincidence, Escherichia coli would be not detected after 24 h of administration in big tissue cage and in small one. The bacteria quickly decreased in initiation stage of contacting enrofloxacin in tissue cages. The dosage of enrofloxacin should be decreased if the half-life was longer by analyzing.