载脂蛋白E基因多态性对血脂康胶囊抑制炎症作用的影响

Effects of ApoE Gene Polymorphism on Anti-inflammatory Action of Xuezhikang Capsule

目的探讨载脂蛋白E(apolipoprotein E,ApoE)基因多态性对血脂康胶囊抑制炎症作用的影响。方法采用基因测序法检测102例高脂血症患者和100名健康体检者ApoE基因多态性和血清肿瘤坏死因子α(tumor necrosis factoralpha,TNF-α)、白介素-6(interleukin 6,IL-6)、高敏C反应蛋白(high sensitivity Creactive protein,hs-CRP)等炎症因子表达,并检测血脂康胶囊干预6周后高脂血症患者TNF-α、IL-6、hs-CRP等炎症因子的变化,分析ApoE基因多态性与各炎症因子变化值之间的关系。结果健康体检者E3/3基因型(86%,86/100)显著高于高脂血症组(62.7%,64/102)、E3/4基因型(6%,6/100)显著低于高脂血症组(21.6%,22/102)(P<0.05);高脂血症组治疗前TNF-α、IL-6、hs-CRP水平明显高于健康体检组(P<0.05),E3/4+E4/4型TNF-α高于E3/3型(P<0.05)、IL-6高于E2/E2+E2/E3型(P<0.05)、hs-CRP高于E2/E2+E2/E3型和E3/E3型(P<0.05),而E2/E2+E2/E3型和E3/E3型TNF-α、IL-6、hs-CRP三者比较,差异均无统计学意义;血脂康干预6周后,高脂血症组TNF-α、IL-6、hs-CRP等水平较治疗前均显著降低(P<0.05) ,但TNF-α、IL-6水平仍显著高于健康体检组(P<0.05),但E2/E2+E2/E3型、E3/E3型和E3/4+E4/4型3组TNF-α、IL-6、hs-CRP治疗前后差值比较,差异均无统计学意义(P>0.05)。结论 ApoE基因型在高脂血症患者和健康人群间分布存在差异;高脂血症者存在慢性炎症反应,含ε4等位基因者更为明显;血脂康具有抗炎作用,但ApoE基因多态性不影响其抗炎疗效。

Objective To study the effects of ApoE gene polymorphism on antiinflammatory ac tion of Xuezhikang Capsule. Methods One hundred and two patients with hyperlipidemia （as the treated group）and one hundred healthy volunteers （as the control group）were enrolled in the casecontrol study. Total DNA of the peripheral blood was extracted and ApoE genotypes were determined by PCR sequence analysis. The serum levels of tumor necrosis factor alpha （TNFoL）, interleukin 6 （IL-6）, and high sensi tivity C reactive protein （hsCRP）were measured in all subjects. The changes of TNF-a, IL-6, and hsCRP were detected before and after 6week Xuezhikang Capsule treatment, thus analyzing the correlation between ApoE gene polymorphism and changes of each inflammatory factor. Results The frequency of E3f3 genotype was 86% （86/100 cases）in the control group, significantly higher than that of the treated group （62.7%, 64/102 cases）. The frequency of E3/4 genotype was 6% （6/100 cases）in the control group, significantly lower than that of the treated group （21.6%, 22/102 cases; both P 〈0.05）. Com pared with the control group, the serum levels of TNF-a, IL-6, and hsCPR were higher in the treated group before treatment （P 〈0.05）. In hyperlipidemia patients with E3/4 ＋ E4/4 genotype, the serum level of TNF-a was higher than that of E3/3 genotype （ P 〈0.05） ; the serum level of IL-6 was higher than that of E2/E2 ＋ E2/E3 genotype （ P 〈0.05） ; the serum level of hsCRP was higher than that of E2/E2 ＋ E2/E3 and E3/E3 genotype （P 〈0.05）.But there was no statistical difference in the serum levels of TNF-a, IL-6, or hsCPR between E3/3 and E2/E2 ＋ E2/E3 genotype. After 6week intervention of Xuezhikang Capsule, the serum levels of TNF-a, IL-6, and hsCRP were lower in the treated group （P 〈0.05）, but the serum lev els of TNF-a and IL-6 were still higher than those of the control group （P 〈0.05）. But there was no statisti cal difference in the decrement of TNF-a, IL-6, or hsCRP among E2/E2 ＋ E2/E3, E3/E3, or E3/4 ＋ E4/4 geno types （ P 〉0.05）. Conclusions The distribution of ApoE gene polymorphism is different between the hyperlipi demia patients and the healthy people. Chronic inflammatory reactions exist in hyperlipidemia patients, espe cially in those with ε4 allele. Xuezhikang Capsule showed antiinflammatory effects, but ApoE gene polymor phism did not affect its effects.