伤科接骨片对兔下颌骨缺损修复中骨保护素及配体基因表达的影响

Effects of Shangke Jiegu Tablet on the Gene Expressions of Osteoprotegerin and Osteoprotegerin Ligand in the Repairing Process of Mandibular Defect Rabbits

目的探讨伤科接骨片在下颌骨缺损修复中的作用机制。方法健康雄性新西兰兔72只,随机分为正常对照组(24只)、模型组(24只)和伤科接骨片组(24只),建立下颌骨缺损模型。正常对照组、模型组动物给予正常饲料,伤科接骨片组动物给予伤科接骨片饲料。分别于建模后第7、14、28、56天处死相同例数动物(n=6),收集下颌骨缺损处骨组织块。采用RT-PCR技术检测骨保护素(osteoprotegerin,OPG)、骨保护素配体(osteoprotegerin ligand,OPGL)基因表达,免疫组化检测骨组织染色强度和面积。结果与正常对照组比较,模型组兔下颌骨组织OPGmRNA表达在建模后第7天显著上调(P<0.05),OPGL mRNA表达在建模后第14天显著上调(P<0.05);与模型组比较,伤科接骨片组建模后第14、28、56天OPGmRNA表达均上调明显(P<0.05),骨组织阳性染色更强,面积更广,建模后第14天OPGL mRNA表达下调明显(P<0.05),骨组织阳性染色更弱,面积更小,OPG mRNA/OPGL mRNA比值在建模后第14、28、56天明显上调(P<0.05)。结论伤科接骨片促进下颌骨缺损修复的作用可能与其调节OPGmRNA、OPGL mRNA表达水平有关。

Objective To explore the mechanism of Shangke Jiegu Tablet （SJT）in repairing the mandibular defect. Methods Totally 72 healthy male New Zealand rabbits were randomly divided into the normal control group （ n =24）, the model group （ n =24）, and the SJT group （ n =24）. Then the mandibular defect model was established. Animals in the normal control group and the model group were fed with normal forage, while those in the SJT group were fed with SJT forage. On the day 7, 14,28, and 56 after model establishment, 6 rabbits were killed in each group. The bone was collected from the mandibular defect. The gene expressions of osteoprotegerin （OPG） and osteoprotegerin ligand （OPGL） were detected by means of RT-PCR. The positive dyeing strength and area of the bone tissue were detected by means of immunohistochemical technique. Results Compared with the normal control group, the degree of OPGmRNA expression was remarkably up-regulated on day 7 after model establishment （P 〈0.05）and the degree of OPGLmRNA expression was remarkably up-regulated on day 14 after model establishment （P 〈0.05）in the model group. Compared with the model group, the degree of OPGmRNA expression was remarkably up-regulated （P 〈0.05）, and the positive dyeing strength and area of bone tissue were stronger and broader on day 14, 28, and 56 after model establishment in the SJT group. The degree of OPGLmRNA expression was remarkably down-regulated （P 〈0.05）, and the positive dyeing strength and area of bone tissue were weaker and smaller on day 14 after model establishment in the SJT aroup.The ratio of OPGmRNA/OPGLmRNA was remarkably up-regulated on day 14, 28, and 56 after model establishment （P 〈0.05）. Conclusion The effect mechanism of promoting mandibular defect repairing by SJT may be correlated to regulating the expressions of OPGmRNA and OPGLmRNA.