猪源枯草芽孢杆菌喷雾干燥工艺的优化

Optimization of procedure for spray drying of Bacillus subtilis from swine origin

目的优化猪源枯草芽孢杆菌喷雾干燥工艺,提高菌体喷雾干燥后的存活率。方法应用SAS V 8.0统计分析软件中的二次正交旋转组合设计方案进行试验设计,分析各因素对Y值的效应关系;应用优化的喷雾干燥工艺进行3次重复喷雾干燥试验,验证预测结果的准确性;并检测芽孢率对菌体喷雾干燥存活率的影响。结果优化的喷雾干燥工艺中各条件的最佳参数为:出口温度90℃,入口温度150℃,保护剂浓度20%,入料速度1 200 ml/h;应用优化的喷雾干燥工艺进行3次重复喷雾干燥试验,菌体平均存活率为75.7%,与预测值(77%)基本相符;芽孢率对菌体喷雾干燥存活率影响较大。结论优化了猪源枯草芽孢杆菌的喷雾干燥工艺,为其工业化生产奠定了基础。

Objective To optimize the procedure for spray drying of Bacillus subtilis from swine origin and increase the survival rate of bacteria after spray drying.Methods The test was designed by using the Quadratic Orthogonal Rotation Combination Design in SAS V8.0 software,and the effect of various factors on response value was analyzed.Three repeat spray drying tests were performed by the optimized procedure to verify the accuracy of predicted result.The effect of germ formation rate on survival rate of bacteria after spray drying was evaluated.Results The optimal outlet air temperature,inlet air temperature,stabilizer concentration and liquid feed rate were 90 ℃,150 ℃,20% and 1 200 ml / h respectively.The mean survival rate of bacteria in three repeat spray drying tests was 75.7%,which was basically consistent with the predicted value（77%）.The germ formation rate showed significant effect on the survival rate of bacteria after spray drying.Conclusion The procedure for spray drying of Bacillus subtilis from swine origin was successfully optimized,which laid a foundation of industrial production of B.subtilis spray.