大麻素受体在溃疡性结肠炎患者血清和肠组织中的表达

Expressions of cannabinoid receptor in serum and intestinal tissue with ulcerative colitis

目的检测大麻素受体1(CNR1)和大麻素受体2(CNR2)在溃疡性结肠炎(UC)患者血清和肠组织中的表达,探讨其与UC的关系及临床意义。方法连续收集35例UC(UC组)、20例已排除炎症性肠病的普通肠炎患者(疾病对照组)和15例肠黏膜病理正常者(正常对照组),用酶联免疫吸附(ELISA)法检测其血清中CNR1、CNR2、C反应蛋白(CRP)和白介素(IL)-10表达水平;免疫组化法检测肠道黏膜中CNR1和CNR2的表达情况。结果 UC患者血清中CNR1、CNR2含量明显高于两个对照组(P<0.05)。UC活动期血清中CNR1、CNR2、IL-10表达量要低于缓解期,而CRP高于缓解期(P<0.01)。UC肠道黏膜CNR1、CNR2表达率明显高于普通肠炎黏膜以及正常肠黏膜(P<0.05),而后两者相比差异无统计学意义。结论 CNR在UC中表达显著上调,表明CNR对UC的发生发展可能起着重要作用。

Objective To detect the expressions of cannabinoid receptor 1 （ CNR1 ） and CNR2 in patients with ul- cerative colitis （UC） in serum and intestinal tissue; to explore its relationship with the UC and its clinical signifi- cance. Methods 35 patients with ulcerative colitis （UC）, 20 patients with other non-IBD gastrointestinal conditions（ disease control group） and 15 pathology of the normal intestinal mucosa（normal control group）, its the levels of serum CNR1, CNR2, C-reactive protein （CRP） and interleukin-10 （IL-10） were measured by enzyme-linked im- munosorbent assay （ ELISA ） . The immunohistochemical method was used to detect the expression of these intestinal mucosa CNR1 and CNR2. Results Compared with non-IBD gastrointestinal conditions and normal controls, the levels of serum CNR1, CNR2 in UC patients were significantly increased. UC activity of serum CNR1, CNR2 and IL- 10 expression level is lower than that in remission period, and CRP is higher than patients in remission period （P 〈 0. 01 ）. The intestinal mucosa of UC CNR1, CNR2 expression was significantly higher than the ordinary of enteritis mucosa and pathological normal intestinal mucosa （P 〈 0.05 ）, the latter two showed no significant difference. Conclusion CNR in the UC patients expresses significant increase, which suggests that CNR may play an important role in occurrence and development of UC.