miR-24和miR-22在胃癌中的表达及其临床意义

Expression of miR-24 and miR-22 in gastric cancer and their clinical significance

目的探讨miR-24和miR-22在胃癌细胞与组织中的表达及其与胃癌临床病理特征之间的关系。方法应用实时荧光定量PCR(qPCR)检测miR-24和miR-22在人正常胃黏膜细胞株(GES-1)、4株人胃癌细胞株以及胃癌组织和相应的癌旁胃黏膜组织中的表达,分析miR-24和miR-22在胃癌中的表达及其与胃癌临床病理特征之间的关系。结果与GES-1比较,miR-24在人胃癌细胞株SGC-7901和BGC-823中表达明显升高(P=0.003、P=0.007),而在MGC-803和AGS中表达差异无统计学意义(P=0.304、P=0.120);与GES-1比较,miR-22在SGC-7901中表达明显升高(P=0.000),在MGC-803中表达显著降低(P=0.000),而在AGS和BGC-823中表达差异无统计学意义(P=0.151、P=0.307);胃癌组织中miR-24的表达明显高于其相应的癌旁胃黏膜组织(P=0.005);miR-22在胃癌组织及其相应的癌旁胃黏膜组织中的表达差异无统计学意义(P=0.501);胃癌组织中除miR-24的表达与性别具有显著相关性外(P=0.029),miR-24、miR-22与其他临床病理特征,如年龄、肿块大小、浸润深度、分化程度、Borrmann分型以及淋巴结转移、TNM分期等均无明显相关性。结论 miR-24在人胃癌细胞株SGC-7901和BGC-803以及胃癌组织中表达是上调的,可能在胃癌的发生发展中发挥重要作用。

Objective To investigate the expression of miR-24 and miR-22 in gastric cancer cells and tissues and their relationships with clinicopathologic features. Methods The expression of miR-24 and miR-22 in normal gastric epithelial cell lines （ GES-1 ）, four gastric cancer cell lines, gastric cancer tissues and their matched tumor adjacent tissues were examined by real-time fluorescent quantitative PCR （qPCR）. The relationships between the expression of miR-24, miR-22 and their clinicopathologic features were analyzed. Results Compared with GES-1, the expression levels of miR-24 in gastric cancer cell lines SGC-7901 （ P = 0. 003 ） and BGC-823 （ P = 0. 007 ） were much higher. But miR-24 expression had no statistically significant differences in MGC-803 （P = 0. 304）, AGS （P =0. 120）. The expression level of miR-22 was significantly higher in SGC-7901 than that in GES-1 （P = 0. 000）, and significantly lower in MGC-803 than that in GES-1 （P = 0. 000）. But miR-22 expression had no sta- tistically significant differences in AGS （ P = 0. 151 ）, BGC-823 （ P = 0. 307 ） and GES-1. The expression level of miR-24 in gastric cancer tissues was significantly higher than that in adjacent tissues （P = 0. 005 ）. Up-regulated miR-24 expression was associated with gender in gastric carcinoma patients （P = 0. 029）. There was no statistically significant difference of miR-22 expression in gastric cancer tissues and adjacent tissues （P = 0. 501 ）. No great as- sociation was found between the expression of miR-24, miR-22 and the status of age, size of tumor, cell differentiation, depth of tumor invasion, Borrmann type, TNM staging system and tumor node metastasis stage. Conclusion The expression of miR-24 in gastric cancer cell lines SGC-7901, BGC-803 and gastric cancer tissues is up-regulated, and it may play an important role in the carcinogenesis and development of gastric cancer.