摘要
肉桂酸-4-羟基化酶是苯丙烷途径中继L-苯丙氨酸解氨酶(PAL)之后的第2个关键酶。为了从分子水平上了解C4H基因的功能特性,本文从植物进化的角度出发,根据Genebank中已有的豆科植物C4H氨基酸保守序列为基础,设计引物,通过RT-PCR和cDNA末端快速扩增技术(rapid amplification of cDNA ends,RACE)方法克隆膜荚黄芪C4H基因,对其序列进行分析,为进一步研究其功能奠定基础。
Cinnamic acid-4-hydroxylase(C4H,EC1.14. 13.11)is the second key enzyme in phenylpropanoid metabolic pathway after L-phenylalnine ammonialyase(PAL). In order to understand the functional prop- erties of C4H gene, designed PCR primers that target evolutionarily conserved sequeces of C4H gene in plants and cloned full-length sequences of C4H gene in A. membranaceus by the technique of RT-PCR and RACE, analysised characteristic of sequence.
出处
《延边大学农学学报》
2012年第4期277-281,323,共6页
Agricultural Science Journal of Yanbian University
基金
国家自然科学基金(30860036)
吉林省自然科学基金(201115228)
关键词
膜荚黄芪
C4H
基因克隆
序列分析
Astragalus membranaceus
C4H
gene cloning
sequence analysis