获能和冻融猪精子前顶体蛋白活化机制研究

Studies on mechanism of proacrosin activation of capacitation and frozen-thawed boar spermatozoa

利用SDS-PAGE和Western Blotting的方法,分析冻融和获能处理中猪精子前顶体蛋白的转化过程。结果表明,显示获能组精子荧光区域大于冻融组精子,而小于新鲜精子组;新鲜组精子出现大约45和35kDa分子量的条带,而冻融和获能组精子都同时出现大约45、35和28kDa分子量的条带。总之,冻融后顶体完整的精子和获能精子Proarosin都能正常活化成α-acrosin、β-acrosin和28kDa,这个途径可作为预测受精的指标。

With SDS-PAGE and Western Blotting methods to analysis proacrosin conversion process of frozen-thawed boar sperm.The results showed that fluorescence region the capacitation group sperm is greater than the frozen-thawed group sperm,while less than fresh group sperm.Fresh group sperm have 45 kDa and 35 kDa two bands,and frozen-thawed and capacitation sperm have 45 kDa,35 kDa and 28 kDa three bands.The results indicated part of the proacrosin are activated into α-acrosin,β-acrosin and 28 kDa protein expression during capacitation and freezing-thawing of boar spermatozoa.In conclusion,acrosomal integrity of boar sperm can be normal fertilization except for 'capacitation-like' sperm after freezing and thawing.