Characterization of thirty-nine polymethoxylated flavonoids （PMFs） in the branches of Murraya paniculata by HPLC-DAD-ESI-MS/MS

试图在 Murraya paniculata (L.) 的分支调查 polymethoxylated flavonoids (PMF ) 杰克。一个敏感 HPLC-DAD-ESI-MS/MS 方法被建立在 M 的分支屏蔽 PMF 的方法。paniculata 基于在由 CID-MS/MS 的积极模式的六个 PMF 标准的分析。结果为 polymethoxylated 黄酮， polymethoxylated 黄烷酮， polymethoxylated chalcones 和 PMF glycosides 的诊断破碎小径分别地被总结。根据 MS 破碎小径， 39 PMF 包括 24 黄酮， 10 黄烷酮或 chalcones 和 5 PMF glycosides 被屏蔽。结果显示了的结论发达分析方法能为在 TCM PMF 屏蔽的化学药品作为一种快速的、有效技术被采用，这提取。

AIM： To investigate the polymethoxylated flavonoids （PMFs） in the branches of Murraya paniculata （L.） Jack. METHODS： A sensitive HPLC-DAD-ESI-MS/MS method was established to screen PMFs in the branches of M. paniculata based on the analysis of six PMF standards in the positive mode by CID-MS/MS. RESULTS： The diagnostic fragmentation pathways for poly- methoxylated flavones, polymethoxylated flavanones, polymethoxylated chalcones and PMF glycosides were summarized, respec- tively. According to the MS fragmentation pathways, 39 PMFs, including 24 flavones, 10 flavanones or chalcones and 5 PMFs gly- cosides were screened. CONCLUSION： The results indicated that the developed analytical method could be employed as a rapid, effective technique for the chemical screening of PMFs in TCMs extracts.