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蛋白免疫共沉淀筛选肾癌A498细胞CXCR4核定位结合蛋白 被引量：2

Co-immunoprecipitation in screening proteins interacting with CXCR4 during nuclear localization in renal cell carcinoma A498 cells
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摘要 目的筛选肾癌A498细胞CXCR4核定位结合蛋白。方法采用蛋白免疫共沉淀实验寻找特异性条带,再通过蛋白质谱分析、生物信息学分析寻找可能与CXCR4结合的蛋白。结果 CXCR4抗体进行蛋白免疫共沉淀,发现有3条特异性条带。选取特异性条带行质谱分析提示可能的蛋白共有36个。将这36个蛋白与CXCR4进行生物信息学分析发现NR1D2、c-src及HSPA8有可能与CXCR4相互作用,参与CXCR4核定位。结论 NR1D2、c-src及HSPA8可能参与了A498细胞CXCR4核定位的过程。 Objective To screen for the proteins interacting with CXCR4 during nuclear localization in renal cell carcinoma （RCC） A498 cells. Methods Specific band in co-immunoprecipitation （Co-IP） experiments was sent for mass spectrometry. With the results of Co-IP experiments and mass spectrometry, the proteins interacting with CXCR4 were determined by bioinformatics analyses. Results Three specific bands were found after Co-IP with anti-CXCR4 antibody, and the results of mass spectrometry of the three specific bands showed 36 proteins possibly interacting with CXCR4. Bioinformatics analyses showed that NR1D2, c-src and HSPA8 might interact with CXCR4 and participate in CXCR4 nuclear localization. Conclusion NR1D2, c-src and HSPA8 might have participated in CXCR4 nuclear localization in RCC A498 cells.
作者 王志向 蔡晨 王林辉 刘冰 杨庆 孙颖浩
机构地区 第二军医大学长海医院泌尿外科 解放军 第二军医大学长海医院特需诊疗科
出处 《第二军医大学学报》 CAS CSCD 北大核心 2013年第1期68-71,共4页 Academic Journal of Second Military Medical University
基金 国家自然科学基金(81272817/H1619)~~
关键词 肾肿瘤 NR1D2 C-SRC HSPA8 CXCR4核定位 免疫共沉淀 kidney neoplasms NR1D2 c-src HSPA8 CXCR4 nuclear localization co-immunoprecipitation
分类号 R737.11 [医药卫生—肿瘤]
  • 相关文献

参考文献14

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二级参考文献15

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共引文献2

同被引文献46

  • 1Pan J, Mestas J, Burdick M D, Phillips R J, Thomas G V,Reckamp K,et al. Stromal derived actor-1 (SDF-1/ CXCL12) and CXCR4 in renal cell carcinoma metasta- sis[J]. Mol Cancer,2006,5:56.
  • 2Haviv Y S,Van houdt W J,Lu B,Curiel D T,Zhu Z B. Transcriptional targeting in renal cancer cell lines via the human CXCR4 promoter[J]. Mol Cancer Ther, 2004,3:687-691.
  • 3Teicher B A, Fricker S P. CXCL12 (SDF-1)/CXCR4 pathway in cancer[J]. Clin Cancer Res, 2010,16 : 2927- 2931.
  • 4Schrader A J, Lechner O, Templin M, Dittmar K E, Machtens S, Mengel M,et al. CXCR4/CXCL12 expres sion and signalling in kidney caneer[J]. Br J Cancer, 2002,86:1250-1256.
  • 5Wang L,Wang L,Yang B,Yang Q,Qiao S,Wang Y,et al. Strong expression of chemokine receptor CXCR4 by renal cell carcinoma cells correlates with metastasis[J].Clin Exp Metastasis,2009,26 : 1049-1054.
  • 6Wang L H,Liu Q,Xu B,Chen W,Yang Q,Wang Z X, et ai. Identification of nuclear localization sequence of CXCR4 in renal cell carcinoma by constructing expres sion plasmids of different deletants[J]. Plasmid,2010, 63..68-72.
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二级引证文献1

第二军医大学学报
2013年 第1期

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