腺相关病毒介导血管内皮生长因子复合无机骨促进骨再生的实验研究

Experimental Study of Guided Bone Regeneration Enhanced by AAV-VEGF Associated with Inorganic Bone Particles

目的:将血管内皮生长因子(VEGF)与腺相关病毒(AAV)重组,并与无机骨颗粒复合,探讨其对骨组织再生的影响。方法:将24只成年新西兰大白兔随机分为2组,在兔颅骨上建立引导骨再生模型。体外构建承载VEGF基因的腺相关病毒,实验组填充复合有AAV-VEGF的无机骨颗粒,对照组仅填充无机骨颗粒。分别在术后2、4、8周对术区进行组织学观察及形态测量学分析。结果:术后4周及8周时,实验组新生骨量和毛细血管数量均较对照组为多,且骨成熟度更高。免疫组化结果显示实验组VEGF蛋白呈强阳性表达。结论:在引导骨再生时配合AAV-VEGF,较单纯植入无机骨能更快、更有效地促进新生骨形成。

Objective： To evaluate the effects of inorganic bone coated with adeno-associated virus-mediated vascular endothelial growth factor （AAV-VEGF） on new bone formation in a guided bone regeneration model system. Methods： Twenty-four adult male New Zealand white rabbits were randomly divided into 2 groups. A guided bone regeneration model was established on the skull of rabbit. The experimental groups were filled with inorganic bone particles loading with AAV-VEGF, while the control groups were filled with inorganic bone particles only. 2, 4 and 8 weeks after the operations, histological and histomorphometric examination were conducted. Results： The experimental groups appeared to contain more bone and more newly formed blood vessels than control groups at 4 and 8 weeks after implantation. Immunohistochemical analysis showed positive expressions of VEGF protein in the experimental group. Conclusion： Applying AAV-VEGF in GBR could improve the angiogenic and osteogenic effects.