乙型肝炎组织精氨酸酶1的表达与其组织学分级的相关性

Association of arginase-1 expression with histological grading and staging in type B hepatitis

目的研究精氨酸酶1(Arg-1)在乙型肝炎组织中的表达及与炎症和纤维化分级的相关性。方法 HBsAg阳性肝穿刺及手术标本118例,按肝炎修正HAI记分法分为G0-G4级和S0-S4期,用Western blot检测Arg-1蛋白在G0,G4,S0,S4组中的表达;用免疫组织化学检测Arg-1在G0,G1,G2,G3,G4及S0,S1,S2,S3,S4各组中的表达。结果 Arg-1在G0、S0组高表达,在G4、S4组表达水平明显降低。Arg-1弥漫表达于G0、S0组肝细胞胞浆和部分肝细胞胞核,门管区肝细胞表达更为明显;随着炎症和纤维化程度增加,Arg-1表达强度逐渐降低,且表达强度与炎症和纤维化分级呈负相关(P<0.01)。结论 Arg-1与乙型肝炎病理生理过程密切相关,可以作为乙型肝炎组织学分级的一项参考指标。

Objective To investigate the relationship between arginase-1 （Arg-1） expression and the histological grading and staging in hepatitis B patients. Methods Tissue specimens were collected from 118 hepatitis B patients with HBsAg-positive. The specimens were divided into groups of GO, G1, G2, G3, G4, SO, S1, S2, S3 and S4 according to HAI scoring method. Western blot was used to detect Arg-1 expression in groups of GO, G4, SO and S4. Immunohistochemical staining was performed to detect Arg-1 expression in all groups. Results There was a high Arg-1 expression in groups of GO and SO. A significant decrease of Arg-1 activity was observed in groups of G4 and S4. Arg-1 in groups of GO and SO was diffusely expressed in cytoplasma of hepatocytes and occasionally of nuclear,especially in periportal hepatocytes. Arg-1 expression was decreased as hepatic inflammation and fibrosis were increased. There was a negative correlation between Arg-1 expression and histological grading and staging（P〈0. 01）. Conclusion Arg-1 expression is closely associated with pathophysiological process of hepatitis B patients, which may be taken as an indicator for histologically grading and staging in the patients with hepatitis B.