摘要
基因组含量又称基因组大小或 DNA1C 值,是指物种配子染色体组所含 DNA 的量。基因组含量是比较和进化基因组学研究的基础。为掌握蔬菜基因组含量变化规律,利用植物 DNA 1C 值数据库和相关文献收集整理了主要蔬菜作物的基因组含量信息,通过统计比较分析得到以下主要结论:(1)流式细胞术(Flow Cytometry,FC)是测定蔬菜基因组含量的最佳方法;(2)睡莲科的莲藕(Nelumbo nucifera)是目前已知的基因组含量最小(0.24 pg)的蔬菜,石蒜科的自然四倍体藠头(Allium chinense)基因组含量最大(32.75 pg);(3)主要蔬菜种类中,石蒜科(19.08 pg)蔬菜平均基因组含量最高,十字花科(0.78pg)和葫芦科(0.78 pg)蔬菜最低;(4)多年生和单子叶蔬菜平均基因组含量分别极显著高于非多年生蔬菜和双子叶蔬菜。
The amount of DNA in an unreplicated gametic chromosome complement is known as the genome size，namely the DNA 1C-value. Information about genome size is of great importance as fundamental reference for research on comparative genomics and genome evolution. To gain a clear understanding on the variation of vegetable genome size，we collected data on genome size from the main vegetables first. Statistical and comparative analysis suggested that flow cytometry（FC）is the best method for genome size estimation；Lotus root（Nelumbo nucifera）has the smallest genome size（0.24 pg）in vegetables while the biggest is natural tetraploid Allium chinense（32.75 pg）；The family with the highest average genome size is Amaryllidaceae（19.08 pg）and the two families with the lowest（both are 0.78 pg）are Brassicaceae and Cucurbitaceae；The vegetable genome size is significantly higher in perennials than that of non-perennials，and the same in monocots than that of dicots.
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第1期135-144,共10页
Acta Horticulturae Sinica
基金
广东省自然科学基金项目(S2011030001410)
广东现代农业产业技术体系建设专项资金项目
