纳米雄黄抑制A431细胞株新生血管的机制研究

Experimental research of realgar nanoparticles on neovascularization suppression of cutaneous squamous cell carcinoma

目的:探讨纳米雄黄抑制人皮肤鳞癌A431细胞新生血管的作用及其相关机理。方法:对皮肤鳞癌A431细胞进行体外培养,分别采用免疫组织化学法和RT-PCR法检测不同浓度纳米雄黄处理后其色素上皮源性因子(PEDF)和VEGF mRNA的表达情况。结果:对照组、5%、15%、25%纳米雄黄组、DDP组、综合组A4 3 1细胞PEDF表达阳性率分别为(21.50±2.28)%、(28.81±2.75)%、(42.23±2.35)%、(56.80±3.80)%、(46.33±2.07)%、(59.82±4.80)%;VEGF mRNA相对表达量为(86.30±6.44)%、(72.45±5.37)%、(52.74±7.21)%、(44.81±4.34)%、(30.92±4.12)%、(18.35±5.09)%。可见,经纳米雄黄作用后,人皮肤鳞癌A431细胞PEDF的生成量明显升高,VEGF转录mRNA水平显著降低,均呈剂量依赖性,与顺铂联合有协同作用。结论:体外实验表明纳米雄黄抑制A431新生血管生成的作用可能与上调PEDF的表达和下调VEGF的表达有关。

Objective：To study the inhibition on growth of blood vessels of human skin squamous cell carcinoma A431 cells with Manometer realgar treatment. Methods： Cultured the skin squamous cell carcinoma A431 cells in vitro,then detect the expression of pigment epithelium- deriwed factor（PEDF） and VEGF mRNA by immunohistochemical staining and RT - PCR method with different concentrations of Manometer realgar processing. Results： In control group,5 % , 15 % ,25 % realgar nanoparticles group, DDP group, integrated group of A431 cells, PEDF expression positive rates were （ 21.50 ± 2.28 ） %, （28.81 ± 2.75 ） %, （42.23 ± 2.35 ） %, （ 56.80 ± 3.80 ） %, （ 46.33 ± 2.07 ） % , （59.82 ± 4.80 ） % ;VEGF mRNA relative expression levels（ （ 86.30 ± 6.44 ） % ,72.45 ± 5.37 ） % , （ 52.74 ± 7.21 ） % , （ 44.81 ± 4.34 ） %, （ 30.92 ± 4.12 ） %, （ 18.35 ± 5.09 ） %. By the nanometer realgar, human skin squamous cell carcinoma A431 cells PEDF significantly higher generation of VEGF transcriptional mRNA levels were significantly lower,showed a dose - dependent with cisplatin. Conclusion： The vitro experiments showed that realgar nanoparticles can inhibit the proliferation of A431 ceils related with increase the expression of PEDF and downregulation of the expression of VEGF to inhibit angiogenesis.