β-榄香烯联合照射对肺腺癌A549细胞凋亡及Livin基因表达的影响

Effect of β-elemene combined with radiotherapy on the expression of Livin mRNA and apoptosis of lung adenocarcinoma cell line A549

目的:检测β-榄香烯乳对肺腺癌A549细胞株放射增敏作用并初步探讨其作用机制。方法:四氮唑蓝比色分析法(MTT法)检测β-榄香烯乳对A549细胞增殖的半数抑制浓度(IC50);将细胞分为对照组(C)、照射组(R)、β-榄香烯乳组(0.1×IC50、0.2×IC50)及β-榄香烯乳联合照射组(0.1×IC50+R、0.2×IC50+R),用不同浓度的β-榄香烯乳处理细胞24h后照射,流式细胞仪检测细胞凋亡率。实时定量PCR技术(real-time PCR)检测细胞Livin基因mRNA表达量。结果:MTT法测得β-榄香烯乳对A549细胞增殖的IC50值为115μg/ml;流式细胞仪检测β-榄香烯乳联合照射组细胞凋亡率明显高于单纯照射组及β-榄香烯乳组(P<0.05);β-榄香烯乳联合照射组细胞Livin基因mRNA表达量较照射组明显下降(P<0.01)。结论:β-榄香烯乳可抑制A549细胞增殖,促进A549细胞凋亡和抑制Livin基因mRNA表达,其放射增敏机制可能与这些作用有关。

Objective： To study the effect of β - elemene combined with radiotherapy on the apoptosis of lung adenocarcinoma cell line A549 and mechanism. Methods： A549 cells were divided into the control group （ C ）, irridation group（ R）, β -elemene group（0.1 ×IC50 and 0.2 × IC50 ） and β -elemene combined with irradiation group （0.1 × IC50 ＋ R and 0.2 ×IC50 ＋ R）. IC50 was obtained through MTF method. Cell apoptosis was detected with flow eytometry. The expression level of Livin mRNA was detected by real - time PCR. Results ： MTT assay showed that IC50 value of A549 cells was 115μg/ml. The flow cytometry confirmed that the apoptosis were significantly increased under the effect of β - elemene, statistically different from those in the control group （ P 〈 0.05 ）. The expression level of Livin mRNA in β - elemene with radiotherapy group was declined significantly, statistically different from those in the control group（ P 〈 0.01 ）. Conclusion：The radiosensitization effect of β -elemene oh A549 cells is to inhibit the proliferation of A549 cells, associated with induction of cell apoptosis and down - regulation of Livin mRNA expression. These effects may be related to radiosensitization mechanism.