β-catenin、Axin及C-myc在肺硬化性血管瘤两种细胞中表达的差异

Different expression pattern of β-catenin,Axin and C-myc in two cell types of pulmonary sclerosing hemangioma

目的:检测肺硬化性血管瘤(pulmonary sclerosing hemangioma,PSH)中β-catenin、Axin及C-myc的表达情况,探讨PSH的肿瘤性质及肿瘤中立方细胞和多角形细胞的分化水平。方法:采用SP免疫组织化学方法检测β-catenin、Axin及C-myc在52例PSH两种细胞中的表达情况。结果:52例PSH中,立方细胞均显示β-catenin连续的细胞膜强阳性表达,而多角形细胞显示β-catenin细胞膜表达减弱或缺失并伴有不同程度细胞浆表达;Axin在立方细胞中表达阳性率92.3%(48/52)显著高于其在多角形细胞中表达阳性率25.0%(13/52,P<0.05);C-myc在立方细胞表达阳性率23.1%(12/52)显著低于其在多角形细胞中表达阳性率82.7%(43/52,P<0.05)。结论:β-catenin、Axin及C-myc在PSH两种细胞中的表达具有显著性差异,两种细胞可能处于不同的分化阶段;C-myc在多角形细胞中高水平表达提示其可能具有较高的增殖活性。

Objective：To investigate the nature of pulmonary sclerosing hemangioma （PSH） and the differentiation stage of the cuboidal cells and polygonal ceils of the tumor. Methods： Immunohistochemistry was used to detect the expression of β - catenin, Axin and C - myc in 52 cases of PSH. Results： Of the 52 PSH cases, the cuboidal cells showed continuous membrane strong expression of β -catenin,while it was predominantly expressed in the cytoplasm and significantly decreased on cell membrane. The positive expression rate of Axin in cuboidal cells was 92.3% （48/ 52） ,which was significantly higher than that in polygonal ceils 25.0% （13/52 ,P 〈 0.05 ）. However, the expression of C - myc in cuboidal cells （23.1%, 12/52） was significantly lower than that in polygonal cells （82.7% ,43/52, P 〈 0.05 ）. Conclusion： Two cell types of PSH showed different expression pattern of [3 - catenin; Axin and C - myc, which suggest that the two cells may be in different stages of differentiation, and the polygonal cells may present a higher proliferative activity.