GP73在肝癌组织中的表达及其与临床病理特征的关系

Golgi protein 73 expression in liver cancer tissue and its clinicopathologic significance

目的:探讨GP73在肝癌组织中的表达及其与患者临床病理特征的关系。方法:采用RT-PCR及Western blot方法检测20例肝癌、相应癌旁组织和10例正常肝组织中GP73 mRNA及蛋白的表达情况,并分析其表达水平与临床病理特性的关系。结果:肝癌组织中,GP73 mRNA与蛋白表达水平均明显高于癌旁组织和正常肝组织(1.25±0.17 vs.0.62±0.06,0.46±0.03;2.20±0.26 vs.0.87±0.07,0.26±0.04)(均P<0.05);GP73 mRNA及蛋白的表达水平在血管有无侵犯及肿瘤分化程度分组间差异有统计学意义,有血管侵犯和肿瘤分化低者GP73表达水平高于无侵犯及分化高者(均P<0.05),而与患者年龄、性别、肿瘤大小、肿瘤数目及血清甲胎蛋白(AFP)水平无明显关系(均P>0.05)。结论:GP73在肝癌组织中高表达,其表达水平与肝癌侵袭性密切相关;它有望成为新的判断肝癌侵袭性及术后转移复发的标志物。

Objective： To investigate the golgi protein 73 （GP73） expression in hepatocellular carcinoma （HCC） tissue and its relations with the clinicopathologic characteristics oft.he HCC patients. Methods： qqae expressions of GP73 mRNA and protein in 20 cases of HCC and their adjacent tissues as well as normal liver tissues of 10 cases were detected by RT-PCR and Western blot method, respectively~ and the relations of its expression level with the clinicopathologic variables of the patients were analyzed.Results： Both expressions of GP73 mRNA and protein in the HCC tissues were significantly higher than those in their adjacent tissues and normal liver tissues （1.25±0.17 vs. 0.62±0.06 and 0.46±0.03; 2.20±0.26 vs. 0.87±0.07 and 0.26±0.04） （all P〈0.05）. The expression levels of GP73 mRNA and protein were significantly different between the groups stratified according to whether vascular invasion was present and the tumor differentiation grade, both of which were significantly higher in patients with vascular invasion and showing lower tumor differentiation grade than those in patients without vascular invasion and showing high tumor differentiation grade （both P〈0.05）. However, GP73 expression showed no obvious relations with the age, gender, tumor size and number and a-fetoprotein （AFP） level of the patients （all P〉0.05）. Conclusion： GP73 expression is increased in HCC tissue, and its expression level is closely related to invasive ability of HCC and therefore, it may serve postoperative recurrence and metastasis of HCC. as a potential marker for estimating the invasive ability and