Th17细胞在骨髓增生异常综合征骨髓组织中的表达及意义

Analysis of Th17 Cells Accumulation from Bone Marrow of Myelodysplastic Syndrome and Its Clinical Signification

目的了解骨髓增生异常综合征(MDS)骨髓Th17细胞、调节性T细胞(Treg)的表达量,以探讨MDS的免疫学机制。方法流式细胞术检测65例MDS患者和18例健康对照骨髓细胞Th17细胞及Treg细胞表达量。ELISA方法分析骨髓血浆细胞因子IL-17、IL-10、TGF-β、IL-21、IL-22水平。结果 MDS患者骨髓Th17细胞及Treg细胞数量均高于健康对照组(P均<0.01)。在MDS亚型中Th17细胞及Treg细胞数量从RA/RAS、RAEB-Ⅰ到RAEB-Ⅱ逐渐增高(P均<0.05)。骨髓细胞因子IL-17、IL-10、TGF-β、IL-21、IL-22水平较对照组显著增高(P均<0.01),在MDS亚型中细胞因子IL-17、IL-10、TGF-β、IL-21、IL-22水平从RA/RAS、RAEB-Ⅰ到RAEB-Ⅱ也逐渐增高(P均<0.05)。结论 MDS骨髓微环境中Th17细胞、Treg细胞表达量均增多,可能促进了MDS病程进展。

Objective To investigate the immunological mechanisms of myelodysplastie syndrome （MDS） pathogenesis through detecting the accumulations of Thl7 cells and Treg cells in marrow tissue of MDS patients. Methods The Thl7 cells and Treg cells were detected from bone marrow of 65 patients with MDS and 18 health donors by flow cytometry. Sera from marrow of patients and from marrow of normal donors were assayed by ELISA for IL - 17, IL - 10, TGF - β, IL - 21 , and IL - 22 levels. Results The number of Thl7 cells and Treg cells accumulated in marrow of MDS patients were higher than that in normal donors （P 〈 0.01 ） , and those number increased gradually with the malignancy accentuate in MDS subtype （from RA/RAS/RAEB - Ⅰ to RAEB - Ⅱ subtype） （P 〈 0.05 ）. Cytokines from sera of marrow, sueh as IL - 17, IL - 10,TGF - β, IL -21 and IL -22 levels were raised in MDS patients compared to controls, from subtype RA/RAS,RAEB - Ⅰ to subtype RAEB - Ⅱ（P 〈 0.05）. Conclusion The increased accumulation of Thl7 cells and Treg cells in marrow microenvironment may be one of the most important mechanism in MDS pathogenesis and development.