人组织激肽释放酶对兔症状性脑血管痉挛后脑氧自由基的影响

Effects of Human Tissue Kallikrein on Cerebral Oxygen Radical in Rabbit with Symptomatic Cerebral Vasospasm

目的探讨人组织激肽释放酶(HTK)对兔症状性脑血管痉挛的脑氧自由基代谢影响。方法双侧颈总动脉结扎后无神经症状日本大耳白兔24只,随机分为3组(n=8):即假手术(Sham)组、蛛网膜下腔出血(SAH)组、人组织激肽释放酶(HTK)组:SAH+静脉滴注HTK10×10-3PNAU/kg,连续静脉给药5天。每组兔子注血前后每日进行神经功能评分,分别在第1次注血前1天(D0)及后第5天(D5)行3D-CTA造影,并在最后一次造影后取新鲜海马组织进行SOD、MDA检测。结果与Sham组比较,SAH组兔基底动脉在SAH后5天痉挛明显(P<0.05),神经功能评分明显升高;与SAH组相比,HTK组在D5时兔基底动脉直径增粗(P<0.05),神经功能评分降低。海马组织SOD、MDA测定:与SAH组相比,HTK组SOD活性较高(P<0.05),与SAH组相比,HTK组MDA含量则较低(P<0.05)。结论人组织激肽释放酶能扩张痉挛的基底动脉,并通过调节氧自由基水平起到脑保护作用。

Objective To investigate the effects of the human tissue kallikrein on cerebral oxygen free radical metabolism in the cerebral vasospasm rabbits. Methods Twenty four Japanese white rabbits with ligation of bilateral common carotid arteries and no neurological deficits were randomized into sham - operation （Sham） group, subarachnoid hemorrhage（SAH） group and human tissue kallikrein （HTK） group. These rabbits were injected of HTK10 × 10-3 PNAU/kg, continuous vein administration 5 days. The neurological scores were observed everyday and cerebral angiography changes were measured twice by 3D -CTA,and hippocampus was removed for detection of MDA and SOD after last CTA examination. Results In SAH group,the basilar artery were significantly vasoconstrictive on 5 day, neurological scores were increased,the SOD activity were decreased and MDA content were increased. In HTK group. SOD activity increased and MDA content decreased. Conclusion Human tissue kallikrein could dilate basilar artery, alleviate neurological changes and adjust the leve of oxygen free radicals obviously in rabbits with symptomatic cerebral vasospasm.